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4kcf

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{{STRUCTURE_4kcf| PDB=4kcf | SCENE= }}
 
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===X-ray Structure of a KijD3 in Complex with FMN and dTDP-3-amino-2,3,6-trideoxy-4-keto-3-methyl-D-glucose===
 
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{{ABSTRACT_PUBMED_23621882}}
 
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==About this Structure==
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==X-ray Structure of a KijD3 in Complex with FMN and dTDP-3-amino-2,3,6-trideoxy-4-keto-3-methyl-D-glucose==
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[[4kcf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Actinomadura_kijaniata Actinomadura kijaniata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KCF OCA].
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<StructureSection load='4kcf' size='340' side='right'caption='[[4kcf]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4kcf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinomadura_kijaniata Actinomadura kijaniata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KCF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.098&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKM:[(2R,4S,6R)-4-AZANYL-4,6-DIMETHYL-5,5-BIS(OXIDANYL)OXAN-2-YL]+[[(2R,3S,5R)-5-[5-METHYL-2,4-BIS(OXIDANYLIDENE)PYRIMIDIN-1-YL]-3-OXIDANYL-OXOLAN-2-YL]METHOXY-OXIDANYL-PHOSPHORYL]+HYDROGEN+PHOSPHATE'>AKM</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kcf OCA], [https://pdbe.org/4kcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kcf RCSB], [https://www.ebi.ac.uk/pdbsum/4kcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kcf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/B3TMR1_ACTKI B3TMR1_ACTKI]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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KijD3 is a flavin-dependent N-oxygenase implicated in the formation of the nitro-containing sugar d-kijanose, found attached to the antibiotic kijanimicin. For this investigation, the structure of KijD3 in complex with FMN and its dTDP-sugar substrate was solved to 2.1 A resolution. In contrast to the apoenzyme structure, the C-terminus of the protein becomes ordered and projects into the active site cleft [Bruender, N. A., Thoden, J. B., and Holden, H. M. (2010) Biochemistry 49, 3517-3524]. The amino group of the dTDP-aminosugar that is oxidized is located 4.9 A from C4a of the flavin ring. The model provides a molecular basis for understanding the manner in which KijD3 catalyzes its unusual chemical transformation.
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==Reference==
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Active Site Architecture of a Sugar N-Oxygenase.,Thoden JB, Branch MC, Zimmer AL, Bruender NA, Holden HM Biochemistry. 2013 Apr 30. PMID:23621882<ref>PMID:23621882</ref>
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<ref group="xtra">PMID:023621882</ref><references group="xtra"/><references/>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4kcf" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Actinomadura kijaniata]]
[[Category: Actinomadura kijaniata]]
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[[Category: Branch, M C.]]
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[[Category: Large Structures]]
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[[Category: Bruender, N A.]]
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[[Category: Branch MC]]
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[[Category: Holden, H M.]]
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[[Category: Bruender NA]]
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[[Category: Thoden, J B.]]
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[[Category: Holden HM]]
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[[Category: Zimmer, A L.]]
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[[Category: Thoden JB]]
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[[Category: 6-trideoxy-4-keto-3-methyl-d-glucose]]
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[[Category: Zimmer AL]]
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[[Category: Class d flavin containing monooxygenase]]
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[[Category: Dtdp-3-amino-2]]
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[[Category: Fad]]
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[[Category: Fatty acyl-coa dehydrogenase family]]
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[[Category: Flavoprotein]]
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[[Category: Fmn]]
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[[Category: Kijanimicin]]
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[[Category: Kijanose]]
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[[Category: Kijd3]]
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[[Category: N-oxygenase]]
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[[Category: Nucleotide linked sugar]]
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[[Category: Oxidoreductase]]
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Current revision

X-ray Structure of a KijD3 in Complex with FMN and dTDP-3-amino-2,3,6-trideoxy-4-keto-3-methyl-D-glucose

PDB ID 4kcf

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