3w1k

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{{STRUCTURE_3w1k| PDB=3w1k | SCENE= }}
 
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===Crystal structure of the selenocysteine synthase SelA and tRNASec complex===
 
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{{ABSTRACT_PUBMED_23559248}}
 
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==Function==
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==Crystal structure of the selenocysteine synthase SelA and tRNASec complex==
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[[http://www.uniprot.org/uniprot/SELA_AQUAE SELA_AQUAE]] Converts seryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis (By similarity).
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<StructureSection load='3w1k' size='340' side='right'caption='[[3w1k]], [[Resolution|resolution]] 7.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3w1k]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5] and [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis_MB4 Caldanaerobacter subterraneus subsp. tengcongensis MB4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3W1K FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 7.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3w1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w1k OCA], [https://pdbe.org/3w1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3w1k RCSB], [https://www.ebi.ac.uk/pdbsum/3w1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3w1k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SELA_AQUAE SELA_AQUAE] Converts seryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 21st amino acid, selenocysteine (Sec), is synthesized on its cognate transfer RNA (tRNA(Sec)). In bacteria, SelA synthesizes Sec from Ser-tRNA(Sec), whereas in archaea and eukaryotes SepSecS forms Sec from phosphoserine (Sep) acylated to tRNA(Sec). We determined the crystal structures of Aquifex aeolicus SelA complexes, which revealed a ring-shaped homodecamer that binds 10 tRNA(Sec) molecules, each interacting with four SelA subunits. The SelA N-terminal domain binds the tRNA(Sec)-specific D-arm structure, thereby discriminating Ser-tRNA(Sec) from Ser-tRNA(Ser). A large cleft is created between two subunits and accommodates the 3'-terminal region of Ser-tRNA(Sec). The SelA structures together with in vivo and in vitro enzyme assays show decamerization to be essential for SelA function. SelA catalyzes pyridoxal 5'-phosphate-dependent Sec formation involving Arg residues nonhomologous to those in SepSecS. Different protein architecture and substrate coordination of the bacterial enzyme provide structural evidence for independent evolution of the two Sec synthesis systems present in nature.
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==About this Structure==
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Decameric SelA*tRNA(Sec) ring structure reveals mechanism of bacterial selenocysteine formation.,Itoh Y, Brocker MJ, Sekine S, Hammond G, Suetsugu S, Soll D, Yokoyama S Science. 2013 Apr 5;340(6128):75-8. doi: 10.1126/science.1229521. PMID:23559248<ref>PMID:23559248</ref>
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[[3w1k]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus_vf5 Aquifex aeolicus vf5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W1K OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023559248</ref><references group="xtra"/><references/>
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</div>
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[[Category: Aquifex aeolicus vf5]]
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<div class="pdbe-citations 3w1k" style="background-color:#fffaf0;"></div>
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[[Category: Itoh, Y.]]
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[[Category: Sekine, S.]]
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==See Also==
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[[Category: Yokoyama, S.]]
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*[[Selenocysteine synthase|Selenocysteine synthase]]
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[[Category: Homodecamer]]
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*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
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[[Category: Non-canonical trna]]
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== References ==
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[[Category: Pentamer of dimer]]
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<references/>
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[[Category: Protein-rna complex]]
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__TOC__
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[[Category: Selenium metabolism]]
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</StructureSection>
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[[Category: Selenocysteine synthesis]]
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[[Category: Aquifex aeolicus VF5]]
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[[Category: Transferase-rna complex]]
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[[Category: Caldanaerobacter subterraneus subsp. tengcongensis MB4]]
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[[Category: Large Structures]]
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[[Category: Itoh Y]]
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[[Category: Sekine S]]
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[[Category: Yokoyama S]]

Current revision

Crystal structure of the selenocysteine synthase SelA and tRNASec complex

PDB ID 3w1k

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