1f2s

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{{STRUCTURE_1f2s| PDB=1f2s | SCENE= }}
 
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===CRYSTAL STRUCTURE OF THE COMPLEX FORMED BETWEEN BOVINE BETA-TRYPSIN AND MCTI-A, A TRYPSIN INHIBITOR OF SQUASH FAMILY AT 1.8 A RESOLUTION===
 
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{{ABSTRACT_PUBMED_10524768}}
 
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==Function==
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==CRYSTAL STRUCTURE OF THE COMPLEX FORMED BETWEEN BOVINE BETA-TRYPSIN AND MCTI-A, A TRYPSIN INHIBITOR OF SQUASH FAMILY AT 1.8 A RESOLUTION==
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[[http://www.uniprot.org/uniprot/ITR2_MOMCH ITR2_MOMCH]] Inhibits trypsin.
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<StructureSection load='1f2s' size='340' side='right'caption='[[1f2s]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1f2s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1mcu 1mcu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F2S FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.79&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f2s OCA], [https://pdbe.org/1f2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f2s RCSB], [https://www.ebi.ac.uk/pdbsum/1f2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f2s ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRY1_BOVIN TRY1_BOVIN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f2/1f2s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f2s ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The stoichiometric complex formed between bovine beta-trypsin and Momordica charantia, Linn. Cucurbitaceae trypsin inhibitor A (MCTI-A) was crystallized and its X-ray crystal structure was refined to a final R value of 0.179 using data of 7.0- to 1.8-A resolution. Combination with results on the complex of MCTI-A with porcine trypsin gives the sequence of MCTI-A definitely, of which 13 residues are conserved compared with other squash family trypsin inhibitors. Its spatial structure and the conformation of its primary binding segment from Cys3I (P3) to Glu7I (P3'), which contains a reactive scissile bond Arg5I C-Ile6I N, were found to be very similar to the other squash family proteinase inhibitors.
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==About this Structure==
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Crystal structure of the complex formed between bovine beta-trypsin and MCTI-A, a trypsin inhibitor of squash family, at 1.8-A resolution.,Zhu Y, Huang Q, Qian M, Jia Y, Tang Y J Protein Chem. 1999 Jul;18(5):505-9. PMID:10524768<ref>PMID:10524768</ref>
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[[1f2s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1mcu 1mcu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F2S OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:010524768</ref><references group="xtra"/><references/>
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</div>
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<div class="pdbe-citations 1f2s" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Trypsin 3D structures|Trypsin 3D structures]]
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*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Large Structures]]
[[Category: Momordica charantia]]
[[Category: Momordica charantia]]
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[[Category: Trypsin]]
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[[Category: Huang Q]]
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[[Category: Huang, Q.]]
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[[Category: Jia Y]]
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[[Category: Jia, Y.]]
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[[Category: Qian M]]
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[[Category: Qian, M.]]
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[[Category: Tang Y]]
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[[Category: Tang, Y.]]
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[[Category: Zhu Y]]
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[[Category: Zhu, Y.]]
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[[Category: Hydrolase-hydrolase inhibitor complex]]
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[[Category: Proteinase-inhibitor complex]]
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[[Category: Trypsin]]
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Current revision

CRYSTAL STRUCTURE OF THE COMPLEX FORMED BETWEEN BOVINE BETA-TRYPSIN AND MCTI-A, A TRYPSIN INHIBITOR OF SQUASH FAMILY AT 1.8 A RESOLUTION

PDB ID 1f2s

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