4bq0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "4bq0" [edit=sysop:move=sysop])
Current revision (11:56, 20 December 2023) (edit) (undo)
 
(5 intermediate revisions not shown.)
Line 1: Line 1:
-
{{STRUCTURE_4bq0| PDB=4bq0 | SCENE= }}
 
-
===Pseudomonas aeruginosa beta-alanine:pyruvate aminotransferase holoenzyme without divalent cations on dimer-dimer interface===
 
-
{{ABSTRACT_PUBMED_23519665}}
 
-
==About this Structure==
+
==Pseudomonas aeruginosa beta-alanine:pyruvate aminotransferase holoenzyme without divalent cations on dimer-dimer interface==
-
[[4bq0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQ0 OCA].
+
<StructureSection load='4bq0' size='340' side='right'caption='[[4bq0]], [[Resolution|resolution]] 1.77&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[4bq0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BQ0 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bq0 OCA], [https://pdbe.org/4bq0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bq0 RCSB], [https://www.ebi.ac.uk/pdbsum/4bq0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bq0 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/BAUA_PSEAE BAUA_PSEAE] Involved in the degradation of beta-alanine. Catalyzes the transfer of the amino group from beta-alanine to pyruvate to yield L-alanine and 3-oxopropanoate. It can also accept both 4-aminobutyrate and (S)-alpha-methylbenzylamine (MBA) as amino-group donors in the presence of pyruvate as an amine acceptor.<ref>PMID:21622750</ref> <ref>PMID:23519665</ref>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The presence of pseudo-symmetry in a macromolecular crystal and its interplay with twinning may lead to an incorrect space-group (SG) assignment. Moreover, if the pseudo-symmetry is very close to an exact crystallographic symmetry, the structure can be solved and partially refined in the wrong SG. Typically, in such incorrectly determined structures all or some of the pseudo-symmetry operations are, in effect, taken for crystallographic symmetry operations and vice versa. A mistake only becomes apparent when the Rfree ceases to decrease below 0.39 and further model rebuilding and refinement cannot improve the refinement statistics. If pseudo-symmetry includes pseudo-translation, the uncertainty in SG assignment may be associated with an incorrect choice of origin, as demonstrated by the series of examples provided here. The program Zanuda presented in this article was developed for the automation of SG validation. Zanuda runs a series of refinements in SGs compatible with the observed unit-cell parameters and chooses the model with the highest symmetry SG from a subset of models that have the best refinement statistics.
-
==Reference==
+
Space-group and origin ambiguity in macromolecular structures with pseudo-symmetry and its treatment with the program Zanuda.,Lebedev AA, Isupov MN Acta Crystallogr D Biol Crystallogr. 2014 Sep 1;70(Pt 9):2430-43. doi:, 10.1107/S1399004714014795. Epub 2014 Aug 29. PMID:25195756<ref>PMID:25195756</ref>
-
<ref group="xtra">PMID:023519665</ref><references group="xtra"/><references/>
+
 
-
[[Category: Pseudomonas aeruginosa pao1]]
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Isupov, M N.]]
+
</div>
-
[[Category: Lebedev, A A.]]
+
<div class="pdbe-citations 4bq0" style="background-color:#fffaf0;"></div>
-
[[Category: Littlechild, J A.]]
+
== References ==
-
[[Category: Sayer, C.]]
+
<references/>
-
[[Category: Westlake, A.]]
+
__TOC__
-
[[Category: Pseudo-translation]]
+
</StructureSection>
-
[[Category: Transferase]]
+
[[Category: Large Structures]]
 +
[[Category: Pseudomonas aeruginosa PAO1]]
 +
[[Category: Isupov MN]]
 +
[[Category: Lebedev AA]]
 +
[[Category: Littlechild JA]]
 +
[[Category: Sayer C]]
 +
[[Category: Westlake A]]

Current revision

Pseudomonas aeruginosa beta-alanine:pyruvate aminotransferase holoenzyme without divalent cations on dimer-dimer interface

PDB ID 4bq0

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4bq0"
Personal tools