4jax

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{{STRUCTURE_4jax| PDB=4jax | SCENE= }}
 
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===Crystal structure of dimeric KlHxk1 in crystal form X===
 
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{{ABSTRACT_PUBMED_23583397}}
 
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==About this Structure==
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==Crystal structure of dimeric KlHxk1 in crystal form X==
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[[4jax]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis_nrrl_y-1140 Kluyveromyces lactis nrrl y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JAX OCA].
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<StructureSection load='4jax' size='340' side='right'caption='[[4jax]], [[Resolution|resolution]] 2.26&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4jax]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JAX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JAX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.26&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jax FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jax OCA], [https://pdbe.org/4jax PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jax RCSB], [https://www.ebi.ac.uk/pdbsum/4jax PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jax ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HXK_KLULA HXK_KLULA]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The bifunctional hexokinase KlHxk1 is a key component of glucose dependent signal transduction in Kluyveromyces lactis. KlHxk1 is phosphorylated in vivo and undergoes ATP-dependent autophosphorylation-inactivation in vitro. This study identifies serine-15 as the site of in vivo phosphorylation and serine-157 as the autophosphorylation-inactivation site. X-ray crystallography of the in vivo phosphorylated enzyme indicates the existence of a ring-shaped symmetrical homodimer carrying two phosphoserine-15 residues. In contrast, small-angle X-ray scattering and equilibrium sedimentation analyses reveal the existence of monomeric phosphoserine-15 KlHxk1 in solution. While phosphorylation at serine-15 and concomitant homodimer dissociation are likely to be involved in glucose signalling, mechanism and putative physiological significance of KlHxk1 inactivation by autophosphorylation at serine-157 remain to be established.
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==Reference==
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In vivo phosphorylation and in vitro autophosphorylation-inactivation of Kluyveromyces lactis hexokinase KlHxk1.,Kettner K, Kuettner EB, Otto A, Lilie H, Golbik RP, Strater N, Kriegel TM Biochem Biophys Res Commun. 2013 Apr 10. pii: S0006-291X(13)00600-1. doi:, 10.1016/j.bbrc.2013.03.121. PMID:23583397<ref>PMID:23583397</ref>
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<ref group="xtra">PMID:023583397</ref><references group="xtra"/><references/>
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[[Category: Hexokinase]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Kluyveromyces lactis nrrl y-1140]]
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</div>
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[[Category: Golbik, R P.]]
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<div class="pdbe-citations 4jax" style="background-color:#fffaf0;"></div>
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[[Category: Kettner, K.]]
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[[Category: Kriegel, T M.]]
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==See Also==
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[[Category: Kuettner, E B.]]
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*[[Hexokinase 3D structures|Hexokinase 3D structures]]
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[[Category: Lilie, H.]]
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== References ==
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[[Category: Otto, A.]]
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<references/>
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[[Category: Strater, N.]]
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__TOC__
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[[Category: Atp binding]]
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</StructureSection>
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[[Category: Hexokinase]]
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[[Category: Kluyveromyces lactis NRRL Y-1140]]
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[[Category: Mig1 binding]]
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[[Category: Large Structures]]
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[[Category: Phosphorylation]]
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[[Category: Golbik RP]]
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[[Category: Ribonuclease h-fold]]
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[[Category: Kettner K]]
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[[Category: Sugar binding]]
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[[Category: Kriegel TM]]
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[[Category: Transferase]]
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[[Category: Kuettner EB]]
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[[Category: Lilie H]]
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[[Category: Otto A]]
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[[Category: Strater N]]

Current revision

Crystal structure of dimeric KlHxk1 in crystal form X

PDB ID 4jax

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