3spd

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{{STRUCTURE_3spd| PDB=3spd | SCENE= }}
 
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===Crystal structure of aprataxin ortholog Hnt3 in complex with DNA===
 
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{{ABSTRACT_PUBMED_21984208}}
 
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==Function==
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==Crystal structure of aprataxin ortholog Hnt3 in complex with DNA==
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[[http://www.uniprot.org/uniprot/APTX_SCHPO APTX_SCHPO]] DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined (By similarity).
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<StructureSection load='3spd' size='340' side='right'caption='[[3spd]], [[Resolution|resolution]] 1.91&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3spd]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SPD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SPD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.912&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3spd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3spd OCA], [https://pdbe.org/3spd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3spd RCSB], [https://www.ebi.ac.uk/pdbsum/3spd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3spd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/APTX_SCHPO APTX_SCHPO] DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Aprataxin is a DNA deadenylase that resolves DNA 5'-AMP termini and reverses abortive DNA ligation. The crystal structures of Schizosaccharomyces pombe aprataxin Hnt3 in its apo form and in complex to dsDNA and dsDNA-AMP reveal how Hnt3 recognizes and processes 5'-adenylated DNA in a structure-specific manner. The bound DNA adopts a 5'-flap conformation that facilitates 5'-AMP access to the active site, where AMP cleavage occurs by a canonical catalytic mechanism.
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==About this Structure==
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Crystal structures of aprataxin ortholog Hnt3 reveal the mechanism for reversal of 5'-adenylated DNA.,Gong Y, Zhu D, Ding J, Dou CN, Ren X, Gu L, Jiang T, Wang DC Nat Struct Mol Biol. 2011 Oct 9;18(11):1297-9. doi: 10.1038/nsmb.2145. PMID:21984208<ref>PMID:21984208</ref>
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[[3spd]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SPD OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:021984208</ref><references group="xtra"/><references/>
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</div>
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<div class="pdbe-citations 3spd" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe 972h-]]
[[Category: Schizosaccharomyces pombe 972h-]]
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[[Category: Ding, J.]]
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[[Category: Ding J]]
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[[Category: Dou, C.]]
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[[Category: Dou C]]
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[[Category: Gong, Y.]]
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[[Category: Gong Y]]
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[[Category: Jiang, T.]]
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[[Category: Jiang T]]
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[[Category: Ren, X.]]
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[[Category: Ren X]]
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[[Category: Wang, D.]]
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[[Category: Wang D]]
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[[Category: Zhu, D.]]
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[[Category: Zhu D]]
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[[Category: Dna binding]]
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[[Category: Dna deadenylase]]
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[[Category: Hit domain]]
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[[Category: Hydrolase-dna complex]]
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[[Category: Zinc finger]]
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Current revision

Crystal structure of aprataxin ortholog Hnt3 in complex with DNA

PDB ID 3spd

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