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3ruj
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| - | {{STRUCTURE_3ruj| PDB=3ruj | SCENE= }} | ||
| - | ===Crystal Structure of N-terminal region of yeast Atg7=== | ||
| - | {{ABSTRACT_PUBMED_22056771}} | ||
| - | == | + | ==Crystal Structure of N-terminal region of yeast Atg7== |
| - | [[http://www.uniprot.org/uniprot/ATG7_YEAST ATG7_YEAST]] E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes.<ref>PMID:9759731</ref> <ref>PMID:8224160</ref> <ref>PMID:7593182</ref> <ref>PMID:10233148</ref> <ref>PMID:10233150</ref> <ref>PMID:11100732</ref> <ref>PMID:11149920</ref> <ref>PMID:12965207</ref> <ref>PMID:15277523</ref> | + | <StructureSection load='3ruj' size='340' side='right'caption='[[3ruj]], [[Resolution|resolution]] 2.10Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3ruj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RUJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RUJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rui|3rui]]</div></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ATG7, APG7, CVT2, YHR171W ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ruj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ruj OCA], [https://pdbe.org/3ruj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ruj RCSB], [https://www.ebi.ac.uk/pdbsum/3ruj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ruj ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/ATG7_YEAST ATG7_YEAST]] E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes.<ref>PMID:9759731</ref> <ref>PMID:8224160</ref> <ref>PMID:7593182</ref> <ref>PMID:10233148</ref> <ref>PMID:10233150</ref> <ref>PMID:11100732</ref> <ref>PMID:11149920</ref> <ref>PMID:12965207</ref> <ref>PMID:15277523</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Autophagy is the degradation of cellular organelles via the lysosomal pathway. The autophagic ubiquitin-like (Ubl) molecule Atg8 is activated by the E1-like enzyme Atg7. As this noncanonical E1 enzyme's domain organization is unique among Ubl-activating E1 enzymes, the structural basis for its interactions with Atg8 and partner E2 enzymes remains obscure. Here we present the structure of the N-terminal domain of Atg7, revealing a unique protein fold and interactions with both autophagic E2 enzymes Atg3 and Atg10. The structure of the C-terminal domain of Atg7 in complex with Atg8 shows the mode of dimerization and mechanism of recognition of Atg8. Notably, the catalytic cysteine residue in Atg7 is positioned close to the C-terminal glycine of Atg8, its target for thioester formation, potentially eliminating the need for large conformational rearrangements characteristic of other E1s. | ||
| - | + | Insights into noncanonical E1 enzyme activation from the structure of autophagic E1 Atg7 with Atg8.,Hong SB, Kim BW, Lee KE, Kim SW, Jeon H, Kim J, Song HK Nat Struct Mol Biol. 2011 Nov 6. doi: 10.1038/nsmb.2165. PMID:22056771<ref>PMID:22056771</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | < | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 3ruj" style="background-color:#fffaf0;"></div> |
| - | [[Category: Hong, S B | + | |
| - | [[Category: Kim, B W | + | ==See Also== |
| - | [[Category: Song, H K | + | *[[Autophagy-related protein 3D structures|Autophagy-related protein 3D structures]] |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Baker's yeast]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Hong, S B]] | ||
| + | [[Category: Kim, B W]] | ||
| + | [[Category: Song, H K]] | ||
[[Category: Atg10]] | [[Category: Atg10]] | ||
[[Category: Atg3]] | [[Category: Atg3]] | ||
[[Category: Protein binding]] | [[Category: Protein binding]] | ||
[[Category: Protein transport]] | [[Category: Protein transport]] | ||
Current revision
Crystal Structure of N-terminal region of yeast Atg7
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