2i9e
From Proteopedia
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| - | [[Image:2i9e.gif|left|200px]]<br /><applet load="2i9e" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2i9e, resolution 2.0Å" /> | ||
| - | '''Structure of Triosephosphate Isomerase of Tenebrio molitor'''<br /> | ||
| - | == | + | ==Structure of Triosephosphate Isomerase of Tenebrio molitor== |
| - | + | <StructureSection load='2i9e' size='340' side='right'caption='[[2i9e]], [[Resolution|resolution]] 2.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[2i9e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Tenebrio_molitor Tenebrio molitor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I9E FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | |
| - | [ | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> |
| - | [[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i9e OCA], [https://pdbe.org/2i9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i9e RCSB], [https://www.ebi.ac.uk/pdbsum/2i9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i9e ProSAT]</span></td></tr> |
| - | [ | + | </table> |
| - | [[ | + | == Function == |
| - | + | [https://www.uniprot.org/uniprot/Q8MPF2_TENMO Q8MPF2_TENMO] | |
| - | + | == Evolutionary Conservation == | |
| - | + | [[Image:Consurf_key_small.gif|200px|right]] | |
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i9/2i9e_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i9e ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A coleopteran triosephosphate isomerase (TIM) from Tenebrio molitor (yellow mealworm beetle) was recombinantly expressed in Escherichia coli and characterized with respect to thermal stability, kinetic parameters and oligomeric state. The enzyme was successfully crystallized and the structure determined by X-ray analysis to 2.0 A resolution. This is the first example of an invertebrate TIM. We compare structural features with known structures of TIMs from microorganisms, plants and vertebrates, and discuss the utility of the Tenebrio TIM sequence, together with several newly sequenced insect TIMs, for molecular phylogenetic analysis. | ||
| - | + | A coleopteran triosephosphate isomerase: X-ray structure and phylogenetic impact of insect sequences.,Knobeloch D, Schmidt A, Scheerer P, Krauss N, Wessner H, Scholz Ch, Kuttner G, von Rintelen T, Wessel A, Hohne W Insect Mol Biol. 2010 Feb;19(1):35-48. Epub 2009 Oct 22. PMID:19849721<ref>PMID:19849721</ref> | |
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 2i9e" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Tenebrio molitor]] | ||
| + | [[Category: Hoehne W]] | ||
| + | [[Category: Krauss N]] | ||
| + | [[Category: Scheerer P]] | ||
| + | [[Category: Schmidt A]] | ||
| + | [[Category: Wessner H]] | ||
Current revision
Structure of Triosephosphate Isomerase of Tenebrio molitor
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