4ibn

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{{STRUCTURE_4ibn| PDB=4ibn | SCENE= }}
 
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===Crystal structure of LC9-RNase H1, a type 1 RNase H with the type 2 active-site motif===
 
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{{ABSTRACT_PUBMED_23523920}}
 
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==About this Structure==
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==Crystal structure of LC9-RNase H1, a type 1 RNase H with the type 2 active-site motif==
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[[4ibn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultured_organism Uncultured organism]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IBN OCA].
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<StructureSection load='4ibn' size='340' side='right'caption='[[4ibn]], [[Resolution|resolution]] 1.62&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4ibn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_organism Uncultured organism]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IBN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IBN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.62&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ibn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ibn OCA], [https://pdbe.org/4ibn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ibn RCSB], [https://www.ebi.ac.uk/pdbsum/4ibn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ibn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/E0X765_9ZZZZ E0X765_9ZZZZ]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of metagenome-derived LC9-RNase H1 was determined. The structure-based mutational analyses indicated that the active site motif of LC9-RNase H1 is altered from DEDD to DEDN. In this motif, the location of the second glutamate residue is moved from alphaA-helix to beta1-strand immediately next to the first aspartate residue, as in the active site of RNase H2. However, the structure and enzymatic properties of LC9-RNase H1 highly resemble those of RNase H1, instead of RNase H2. We propose that LC9-RNase H1 represents bacterial RNases H1 with an atypical DEDN active site motif, which are evolutionarily distinct from those with a typical DEDD active site motif.
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==Reference==
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Crystal structure of metagenome-derived LC9-RNase H1 with atypical DEDN active site motif.,Nguyen TN, You DJ, Kanaya E, Koga Y, Kanaya S FEBS Lett. 2013 May 2;587(9):1418-23. doi: 10.1016/j.febslet.2013.03.020. Epub, 2013 Mar 20. PMID:23523920<ref>PMID:23523920</ref>
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<ref group="xtra">PMID:023523920</ref><references group="xtra"/><references/>
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[[Category: Ribonuclease H]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4ibn" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Uncultured organism]]
[[Category: Uncultured organism]]
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[[Category: Kanaya, E.]]
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[[Category: Kanaya E]]
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[[Category: Kanaya, S.]]
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[[Category: Kanaya S]]
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[[Category: Koga, Y.]]
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[[Category: Koga Y]]
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[[Category: Nguyen, T N.]]
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[[Category: Nguyen T-N]]
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[[Category: You, D J.]]
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[[Category: You D-J]]
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[[Category: Hydrolase]]
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[[Category: Hydrolases']]
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[[Category: Ribonuclease h]]
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[[Category: Rnase h]]
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Current revision

Crystal structure of LC9-RNase H1, a type 1 RNase H with the type 2 active-site motif

PDB ID 4ibn

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