2ibn

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[[Image:2ibn.gif|left|200px]]<br /><applet load="2ibn" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ibn, resolution 1.500&Aring;" />
 
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'''Crystal structure of Human myo-Inositol Oxygenase (MIOX)'''<br />
 
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==About this Structure==
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==Crystal structure of Human myo-Inositol Oxygenase (MIOX)==
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2IBN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=FE:'>FE</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=I1N:'>I1N</scene> and <scene name='pdbligand=CYS:'>CYS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Inositol_oxygenase Inositol oxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.99.1 1.13.99.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IBN OCA].
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<StructureSection load='2ibn' size='340' side='right'caption='[[2ibn]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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[[Category: Homo sapiens]]
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== Structural highlights ==
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[[Category: Inositol oxygenase]]
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<table><tr><td colspan='2'>[[2ibn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IBN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IBN FirstGlance]. <br>
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[[Category: Single protein]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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[[Category: Arrowsmith, C.]]
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=I1N:(2S,3R,4R,5S,6S)-2,3,4,5,6-PENTAHYDROXYCYCLOHEXANONE'>I1N</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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[[Category: Berg, S Van Den.]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ibn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ibn OCA], [https://pdbe.org/2ibn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ibn RCSB], [https://www.ebi.ac.uk/pdbsum/2ibn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ibn ProSAT]</span></td></tr>
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[[Category: Berglund, H.]]
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</table>
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[[Category: Busam, R D.]]
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== Function ==
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[[Category: Collins, R.]]
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[https://www.uniprot.org/uniprot/MIOX_HUMAN MIOX_HUMAN]
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[[Category: Edwards, A.]]
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== Evolutionary Conservation ==
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[[Category: Ehn, M.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Flodin, S.]]
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Check<jmol>
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[[Category: Flores, A.]]
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<jmolCheckbox>
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[[Category: Graslund, S.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ib/2ibn_consurf.spt"</scriptWhenChecked>
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[[Category: Hallberg, B M.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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[[Category: Hammarstrom, M.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Hogbom, M.]]
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</jmolCheckbox>
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[[Category: Holmberg-Schiavone, L.]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ibn ConSurf].
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[[Category: Johansson, I.]]
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<div style="clear:both"></div>
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[[Category: Karlberg, T.]]
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<div style="background-color:#fffaf0;">
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[[Category: Kotenyova, T.]]
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== Publication Abstract from PubMed ==
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[[Category: Nilsson-Ehle, P.]]
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Altered inositol metabolism is implicated in a number of diabetic complications. The first committed step in mammalian inositol catabolism is performed by myo-inositol oxygenase (MIOX), which catalyzes a unique four-electron dioxygen-dependent ring cleavage of myo-inositol to D-glucuronate. Here, we present the crystal structure of human MIOX in complex with myo-inosose-1 bound in a terminal mode to the MIOX diiron cluster site. Furthermore, from biochemical and biophysical results from N-terminal deletion mutagenesis we show that the N terminus is important, through coordination of a set of loops covering the active site, in shielding the active site during catalysis. EPR spectroscopy of the unliganded enzyme displays a two-component spectrum that we can relate to an open and a closed active site conformation. Furthermore, based on site-directed mutagenesis in combination with biochemical and biophysical data, we propose a novel role for Lys(127) in governing access to the diiron cluster.
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[[Category: Nordlund, P.]]
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[[Category: Nyman, T.]]
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[[Category: Ogg, D.]]
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[[Category: Persson, C.]]
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[[Category: SGC, Structural Genomics Consortium.]]
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[[Category: Sagemark, J.]]
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[[Category: Stenmark, P.]]
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[[Category: Sundstrom, M.]]
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[[Category: Thorsell, A G.]]
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[[Category: Uppenberg, J.]]
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[[Category: Weigelt, J.]]
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[[Category: CYS]]
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[[Category: FE]]
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[[Category: I1N]]
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[[Category: SO4]]
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[[Category: diiron]]
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[[Category: inositol]]
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[[Category: reductase]]
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[[Category: sgc]]
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[[Category: structural genomics]]
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[[Category: structural genomics consortium]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:50:56 2008''
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Structural and biophysical characterization of human myo-inositol oxygenase.,Thorsell AG, Persson C, Voevodskaya N, Busam RD, Hammarstrom M, Graslund S, Graslund A, Hallberg BM J Biol Chem. 2008 May 30;283(22):15209-16. Epub 2008 Mar 24. PMID:18364358<ref>PMID:18364358</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2ibn" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Arrowsmith C]]
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[[Category: Berglund H]]
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[[Category: Busam RD]]
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[[Category: Collins R]]
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[[Category: Edwards A]]
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[[Category: Ehn M]]
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[[Category: Flodin S]]
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[[Category: Flores A]]
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[[Category: Graslund S]]
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[[Category: Hallberg BM]]
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[[Category: Hammarstrom M]]
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[[Category: Hogbom M]]
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[[Category: Holmberg-Schiavone L]]
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[[Category: Johansson I]]
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[[Category: Karlberg T]]
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[[Category: Kotenyova T]]
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[[Category: Nilsson-Ehle P]]
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[[Category: Nordlund P]]
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[[Category: Nyman T]]
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[[Category: Ogg D]]
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[[Category: Persson C]]
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[[Category: Sagemark J]]
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[[Category: Stenmark P]]
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[[Category: Sundstrom M]]
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[[Category: Thorsell AG]]
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[[Category: Uppenberg J]]
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[[Category: Van Den Berg S]]
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[[Category: Weigelt J]]

Current revision

Crystal structure of Human myo-Inositol Oxygenase (MIOX)

PDB ID 2ibn

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