4j4m

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:00, 6 November 2024) (edit) (undo)
 
(5 intermediate revisions not shown.)
Line 1: Line 1:
-
{{STRUCTURE_4j4m| PDB=4j4m | SCENE= }}
 
-
===Crystal structure of TM-1, a Trimeresurus mucrosquamatus venom metalloproteinase===
 
-
{{ABSTRACT_PUBMED_23732127}}
 
-
==About this Structure==
+
==Crystal structure of TM-1, a Trimeresurus mucrosquamatus venom metalloproteinase==
-
[[4j4m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Protobothrops_mucrosquamatus Protobothrops mucrosquamatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J4M OCA].
+
<StructureSection load='4j4m' size='340' side='right'caption='[[4j4m]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[4j4m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Protobothrops_mucrosquamatus Protobothrops mucrosquamatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J4M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J4M FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j4m OCA], [https://pdbe.org/4j4m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j4m RCSB], [https://www.ebi.ac.uk/pdbsum/4j4m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j4m ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/VM1T1_PROMU VM1T1_PROMU] Potent fibrinogenolytic protease which cleaves mainly the Aalpha (FGA) and Bbeta (FGB) chains of fibrinogen and slightly the gamma chain (FGG) (PubMed:7488093, PubMed:8193588). Shows preference for substrates having a moderate-size and hydrophobic residue at the P1' position. Preferentially cleaves Ala-|-Leu and Tyr-|-Leu bonds (PubMed:23732127). Is more susceptible to tripeptide inhibitors than TM-3 (AC O57413) (PubMed:9703966).<ref>PMID:23732127</ref> <ref>PMID:7488093</ref> <ref>PMID:8193588</ref> <ref>PMID:9703966</ref>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The crystal structure of TM-1, a P-I class snake-venom metalloproteinase (SVMP) from the Trimeresurus mucrosquamatus venom, was determined at 1.8-A resolution. The structure exhibits the typical feature of SVMPs and is stabilized by three disulfide linkages. The active site shows a deep S1' substrate-binding pocket limited by the non-conserved Pro174 at the bottom. Further comparisons with other SVMPs suggest that the deep S1' site of TM-1 correlates with its high inhibition sensitivity to the endogenous tripeptide inhibitors. Proteolytic specificity analysis revealed that TM-1 prefers substrates having a moderate-size and hydrophobic residue at the P1' position, consistent with our structural observation.
-
==Reference==
+
Crystal structure of a Trimeresurus mucrosquamatus venom metalloproteinase providing new insights into the inhibition by endogenous tripeptide inhibitors.,Chou TL, Wu CH, Huang KF, Wang AH Toxicon. 2013 Sep;71:140-6. doi: 10.1016/j.toxicon.2013.05.009. Epub 2013 May 31. PMID:23732127<ref>PMID:23732127</ref>
-
<ref group="xtra">PMID:023732127</ref><references group="xtra"/><references/>
+
 
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 4j4m" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Protobothrops mucrosquamatus]]
[[Category: Protobothrops mucrosquamatus]]
-
[[Category: Trimerelysin II]]
+
[[Category: Chou TL]]
-
[[Category: Chou, T L.]]
+
[[Category: Huang KF]]
-
[[Category: Huang, K F.]]
+
[[Category: Wang AH]]
-
[[Category: Wang, A H.]]
+
[[Category: Wu CH]]
-
[[Category: Wu, C H.]]
+
-
[[Category: Alpha/beta-mixed fold]]
+
-
[[Category: Endopeptidase]]
+
-
[[Category: Hydrolase]]
+

Current revision

Crystal structure of TM-1, a Trimeresurus mucrosquamatus venom metalloproteinase

PDB ID 4j4m

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4j4m"
Personal tools