4bwa
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==PylRS Y306G, Y384F, I405R mutant in complex with adenylated norbornene== | |
| + | <StructureSection load='4bwa' size='340' side='right'caption='[[4bwa]], [[Resolution|resolution]] 2.45Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4bwa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_mazei Methanosarcina mazei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BWA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BWA FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=N0B:ADENYLATED+NORBORNENE'>N0B</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bwa OCA], [https://pdbe.org/4bwa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bwa RCSB], [https://www.ebi.ac.uk/pdbsum/4bwa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bwa ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PYLS_METMA PYLS_METMA] Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Three for two: By using a Methanosarcina mazei PylRS triple mutant (Y306G, Y384F, I405R) the incorporation of two new exo-norbornene-containing pyrrolysine analogues was achieved. X-ray crystallographic analysis led to the identification of the crucial structural elements involved in substrate recognition by the evolved synthetase. | ||
| - | + | Structural Insights into Incorporation of Norbornene Amino Acids for Click Modification of Proteins.,Schneider S, Gattner MJ, Vrabel M, Flugel V, Lopez-Carrillo V, Prill S, Carell T Chembiochem. 2013 Sep 11. doi: 10.1002/cbic.201300435. PMID:24027216<ref>PMID:24027216</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4bwa" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Methanosarcina mazei]] | ||
| + | [[Category: Carell T]] | ||
| + | [[Category: Fluegel V]] | ||
| + | [[Category: Gattner MJ]] | ||
| + | [[Category: Lopez-Carillo V]] | ||
| + | [[Category: Schneider S]] | ||
| + | [[Category: Vrabel M]] | ||
Current revision
PylRS Y306G, Y384F, I405R mutant in complex with adenylated norbornene
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