3w0l

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{{STRUCTURE_3w0l| PDB=3w0l | SCENE= }}
 
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===The crystal structure of Xenopus Glucokinase and Glucokinase Regulatory Protein complex===
 
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{{ABSTRACT_PUBMED_23733961}}
 
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==Function==
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==The crystal structure of Xenopus Glucokinase and Glucokinase Regulatory Protein complex==
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[[http://www.uniprot.org/uniprot/GCKR_XENLA GCKR_XENLA]] Inhibits glucokinase by forming an inactive complex with this enzyme.
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<StructureSection load='3w0l' size='340' side='right'caption='[[3w0l]], [[Resolution|resolution]] 2.92&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3w0l]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W0L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3W0L FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.92&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F6R:FRUCTOSE+-6-PHOSPHATE'>F6R</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3w0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w0l OCA], [https://pdbe.org/3w0l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3w0l RCSB], [https://www.ebi.ac.uk/pdbsum/3w0l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3w0l ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GCKR_XENLA GCKR_XENLA] Inhibits glucokinase by forming an inactive complex with this enzyme.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glucokinase (GK) is a monomeric allosteric enzyme and plays a pivotal role in blood glucose homeostasis. GK is regulated by GK regulatory protein (GKRP), and indirectly by allosteric effectors of GKRP. Despite the critical roles of GK and GKRP, the molecular basis for the allosteric regulation mechanism of GK by GKRP remains unclear. We determined the crystal structure of Xenopus GK and GKRP complex in the presence of fructose-6-phosphate at 2.9 A. GKRP binds to a super-open conformation of GK mainly through hydrophobic interaction, inhibiting the GK activity by locking a small domain of GK. We demonstrate the molecular mechanism for the modulation of GK activity by allosteric effectors of GKRP. Importantly, GKRP releases GK in a sigmoidal manner in response to glucose concentration by restricting a structural rearrangement of the GK small domain via a single ion pair. We find that GKRP acts as an allosteric switch for GK in blood glucose control by the liver.
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==About this Structure==
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Molecular basis for the role of glucokinase regulatory protein as the allosteric switch for glucokinase.,Choi JM, Seo MH, Kyeong HH, Kim E, Kim HS Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):10171-6. doi:, 10.1073/pnas.1300457110. Epub 2013 Jun 3. PMID:23733961<ref>PMID:23733961</ref>
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[[3w0l]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W0L OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023733961</ref><references group="xtra"/><references/>
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</div>
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[[Category: Glucokinase]]
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<div class="pdbe-citations 3w0l" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Glucokinase Regulatory Protein|Glucokinase Regulatory Protein]]
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*[[Hexokinase 3D structures|Hexokinase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Xenopus laevis]]
[[Category: Xenopus laevis]]
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[[Category: Choi, J M.]]
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[[Category: Choi JM]]
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[[Category: Kim, E.]]
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[[Category: Kim E]]
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[[Category: Kim, H S.]]
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[[Category: Kim HS]]
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[[Category: Kyeong, H H.]]
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[[Category: Kyeong HH]]
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[[Category: Seo, M H.]]
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[[Category: Seo MH]]
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[[Category: Aba sandwich]]
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[[Category: Kinase]]
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[[Category: Sugar binding]]
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[[Category: Transferase-transferase inhibitor complex]]
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[[Category: Typical hexokanse fold]]
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Current revision

The crystal structure of Xenopus Glucokinase and Glucokinase Regulatory Protein complex

PDB ID 3w0l

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