4g3a

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{{STRUCTURE_4g3a| PDB=4g3a | SCENE= }}
 
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===Crystal Structure of MAST/Orbit N-terminal domain===
 
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{{ABSTRACT_PUBMED_23832196}}
 
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==Function==
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==Crystal Structure of MAST/Orbit N-terminal domain==
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[[http://www.uniprot.org/uniprot/CLASP_DROME CLASP_DROME]] Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Required for several aspects of mitotic spindle formation including the formation of the overlapping central spindle microtubules and kinetochore attachment. Required for the incorporation of tubulin subunits at the plus ends of kinetochore microtubules during poleward microtubule flux. Acts antagonistically to Klp10A and Klp67A to maintain metaphase spindle length. Also required for guidance of CNS axons downstream of Abl. May function to identify a subset of microtubules that probe the peripheral growth cone domain, where guidance signals exert their influence on cytoskeletal organization. Also required during oogenesis for the organization of the polarized microtubule network inside the 16-cell cyst that ensures oocyte differentiation.<ref>PMID:10899121</ref> <ref>PMID:10747094</ref> <ref>PMID:12034769</ref> <ref>PMID:12538517</ref> <ref>PMID:15240569</ref> <ref>PMID:15207236</ref> <ref>PMID:16303556</ref> <ref>PMID:15592460</ref> <ref>PMID:16723741</ref>
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<StructureSection load='4g3a' size='340' side='right'caption='[[4g3a]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4g3a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G3A FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.994&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g3a OCA], [https://pdbe.org/4g3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g3a RCSB], [https://www.ebi.ac.uk/pdbsum/4g3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g3a ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mast/Orbit is a nonmotor microtubule-associated protein (MAP) present in Drosophila melanogaster that reportedly binds microtubules at the plus end and is essential for mitosis. Sequence analysis has shown that the N-terminal domain (Mast-M1) resembles TOG domains from the Dis1-TOG family of proteins and stands as a representative of one of the three subclasses of divergent TOG-like domains (TOGL1) that includes human CLASP1. The crystal structure of Mast-M1 has been determined at 2.0 A resolution and provides the first detailed structural description of any TOG-like domain. The structure confirms that Mast-M1 adopts a similar fold to the previously described Dis1-TOG domains of microtubule-binding proteins. A comparison with three known TOG-domain structures from XMAP215/Dis1 family members exposes significant differences between Mast-M1 and other TOG-domain structures in key residues at the proposed tubulin-binding edge.
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==About this Structure==
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The structure of the TOG-like domain of Drosophila melanogaster Mast/Orbit.,De la Mora-Rey T, Guenther BD, Finzel BC Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jul 1;69(Pt 7):723-9. doi:, 10.1107/S1744309113015182. Epub 2013 Jun 27. PMID:23832196<ref>PMID:23832196</ref>
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[[4g3a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G3A OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023832196</ref><references group="xtra"/><references/>
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</div>
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<div class="pdbe-citations 4g3a" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Mora-Rey, T De la.]]
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[[Category: Large Structures]]
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[[Category: Cell cycle]]
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[[Category: De la Mora-Rey T]]
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[[Category: Heat repeat]]
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[[Category: Map protein]]
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[[Category: Microtubule]]
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[[Category: Tog domain]]
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Current revision

Crystal Structure of MAST/Orbit N-terminal domain

PDB ID 4g3a

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