2izy

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:32, 9 May 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2izy.jpg|left|200px]]<br /><applet load="2izy" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2izy, resolution 2.20&Aring;" />
 
-
'''MOLECULAR BASIS OF AKAP SPECIFICITY FOR PKA REGULATORY SUBUNITS'''<br />
 
-
==Overview==
+
==Molecular Basis of AKAP Specificity for PKA Regulatory Subunits==
 +
<StructureSection load='2izy' size='340' side='right'caption='[[2izy]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2izy]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IZY FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2izy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2izy OCA], [https://pdbe.org/2izy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2izy RCSB], [https://www.ebi.ac.uk/pdbsum/2izy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2izy ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/KAP2_MOUSE KAP2_MOUSE] Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iz/2izy_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2izy ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
Localization of cyclic AMP (cAMP)-dependent protein kinase (PKA) by A kinase-anchoring proteins (AKAPs) restricts the action of this broad specificity kinase. The high-resolution crystal structures of the docking and dimerization (D/D) domain of the RIIalpha regulatory subunit of PKA both in the apo state and in complex with the high-affinity anchoring peptide AKAP-IS explain the molecular basis for AKAP-regulatory subunit recognition. AKAP-IS folds into an amphipathic alpha helix that engages an essentially preformed shallow groove on the surface of the RII dimer D/D domains. Conserved AKAP aliphatic residues dominate interactions to RII at the predominantly hydrophobic interface, whereas polar residues are important in conferring R subunit isoform specificity. Using a peptide screening approach, we have developed SuperAKAP-IS, a peptide that is 10,000-fold more selective for the RII isoform relative to RI and can be used to assess the impact of PKA isoform-selective anchoring on cAMP-responsive events inside cells.
Localization of cyclic AMP (cAMP)-dependent protein kinase (PKA) by A kinase-anchoring proteins (AKAPs) restricts the action of this broad specificity kinase. The high-resolution crystal structures of the docking and dimerization (D/D) domain of the RIIalpha regulatory subunit of PKA both in the apo state and in complex with the high-affinity anchoring peptide AKAP-IS explain the molecular basis for AKAP-regulatory subunit recognition. AKAP-IS folds into an amphipathic alpha helix that engages an essentially preformed shallow groove on the surface of the RII dimer D/D domains. Conserved AKAP aliphatic residues dominate interactions to RII at the predominantly hydrophobic interface, whereas polar residues are important in conferring R subunit isoform specificity. Using a peptide screening approach, we have developed SuperAKAP-IS, a peptide that is 10,000-fold more selective for the RII isoform relative to RI and can be used to assess the impact of PKA isoform-selective anchoring on cAMP-responsive events inside cells.
-
==About this Structure==
+
Molecular basis of AKAP specificity for PKA regulatory subunits.,Gold MG, Lygren B, Dokurno P, Hoshi N, McConnachie G, Tasken K, Carlson CR, Scott JD, Barford D Mol Cell. 2006 Nov 3;24(3):383-95. PMID:17081989<ref>PMID:17081989</ref>
-
2IZY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/cAMP-dependent_protein_kinase cAMP-dependent protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.11 2.7.11.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZY OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Molecular basis of AKAP specificity for PKA regulatory subunits., Gold MG, Lygren B, Dokurno P, Hoshi N, McConnachie G, Tasken K, Carlson CR, Scott JD, Barford D, Mol Cell. 2006 Nov 3;24(3):383-95. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17081989 17081989]
+
</div>
-
[[Category: Mus musculus]]
+
<div class="pdbe-citations 2izy" style="background-color:#fffaf0;"></div>
-
[[Category: Single protein]]
+
-
[[Category: cAMP-dependent protein kinase]]
+
-
[[Category: Barford, D.]]
+
-
[[Category: Carlson, C R.]]
+
-
[[Category: Dokurno, P.]]
+
-
[[Category: Gold, M G.]]
+
-
[[Category: Hoshi, N.]]
+
-
[[Category: Lygren, B.]]
+
-
[[Category: Mcconnachie, G.]]
+
-
[[Category: Scott, J D.]]
+
-
[[Category: Tasken, K.]]
+
-
[[Category: acetylation]]
+
-
[[Category: camp]]
+
-
[[Category: camp-binding]]
+
-
[[Category: d/d]]
+
-
[[Category: kinase]]
+
-
[[Category: nucleotide-binding]]
+
-
[[Category: phosphorylation]]
+
-
[[Category: pka]]
+
-
[[Category: rii]]
+
-
[[Category: transferase]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:57:50 2008''
+
==See Also==
 +
*[[CAMP-dependent protein kinase 3D structures|CAMP-dependent protein kinase 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Mus musculus]]
 +
[[Category: Barford D]]
 +
[[Category: Carlson CR]]
 +
[[Category: Dokurno P]]
 +
[[Category: Gold MG]]
 +
[[Category: Hoshi N]]
 +
[[Category: Lygren B]]
 +
[[Category: McConnachie G]]
 +
[[Category: Scott JD]]
 +
[[Category: Tasken K]]

Current revision

Molecular Basis of AKAP Specificity for PKA Regulatory Subunits

PDB ID 2izy

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2izy"
Personal tools