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3vor
From Proteopedia
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| - | {{STRUCTURE_3vor| PDB=3vor | SCENE= }} | ||
| - | ===Crystal Structure Analysis of the CofA=== | ||
| - | {{ABSTRACT_PUBMED_22993096}} | ||
| - | == | + | ==Crystal Structure Analysis of the CofA== |
| - | [[3vor]] is a 1 chain structure with sequence from [ | + | <StructureSection load='3vor' size='340' side='right'caption='[[3vor]], [[Resolution|resolution]] 0.90Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3vor]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VOR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VOR FirstGlance]. <br> | ||
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cofA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vor OCA], [https://pdbe.org/3vor PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vor RCSB], [https://www.ebi.ac.uk/pdbsum/3vor PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vor ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | CofA, a major pilin subunit of colonization factor antigen III (CFA/III), forms pili that mediate small-intestinal colonization by enterotoxigenic Escherichia coli (ETEC). In this study, the crystal structure of an N-terminally truncated version of CofA was determined by single-wavelength anomalous diffraction (SAD) phasing using five sulfurs in the protein. Given the counterbalance between anomalous signal strength and the undesired X-ray absorption of the solvent, diffraction data were collected at 1.5 A resolution using synchrotron radiation. These data were sufficient to elucidate the sulfur substructure at 1.38 A resolution. The low solvent content (29%) of the crystal necessitated that density modification be performed with an additional 0.9 A resolution data set to reduce the phase error caused by the small sulfur anomalous signal. The CofA structure showed the alphabeta-fold typical of type IVb pilins and showed high structural homology to that of TcpA for toxin-coregulated pili of Vibrio cholerae, including spatial distribution of key residues critical for pilin self-assembly. A pilus-filament model of CofA was built by computational docking and molecular-dynamics simulation using the previously reported filament model of TcpA as a structural template. This model revealed that the CofA filament surface was highly negatively charged and that a 23-residue-long loop between the alpha1 and alpha2 helices filled the gap between the pilin subunits. These characteristics could provide a unique binding epitope for the CFA/III pili of ETEC compared with other type IVb pili. | ||
| - | + | Structure of the CFA/III major pilin subunit CofA from human enterotoxigenic Escherichia coli determined at 0.90 A resolution by sulfur-SAD phasing.,Fukakusa S, Kawahara K, Nakamura S, Iwashita T, Baba S, Nishimura M, Kobayashi Y, Honda T, Iida T, Taniguchi T, Ohkubo T Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1418-29. Epub 2012 Sep, 13. PMID:22993096<ref>PMID:22993096</ref> | |
| - | <ref | + | |
| - | [[Category: | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | [[Category: Baba, S | + | </div> |
| - | [[Category: Fukakusa, S | + | <div class="pdbe-citations 3vor" style="background-color:#fffaf0;"></div> |
| - | [[Category: Honda, T | + | |
| - | [[Category: Iida, T | + | ==See Also== |
| - | [[Category: Iwasita, T | + | *[[Pilin 3D structures|Pilin 3D structures]] |
| - | [[Category: Kawahara, K | + | == References == |
| - | [[Category: Kobayashi, Y | + | <references/> |
| - | [[Category: Nakamura, S | + | __TOC__ |
| - | [[Category: Nishimura, M | + | </StructureSection> |
| - | [[Category: Ohkubo, T | + | [[Category: Bacillus coli migula 1895]] |
| - | [[Category: Taniguchi, T | + | [[Category: Large Structures]] |
| + | [[Category: Baba, S]] | ||
| + | [[Category: Fukakusa, S]] | ||
| + | [[Category: Honda, T]] | ||
| + | [[Category: Iida, T]] | ||
| + | [[Category: Iwasita, T]] | ||
| + | [[Category: Kawahara, K]] | ||
| + | [[Category: Kobayashi, Y]] | ||
| + | [[Category: Nakamura, S]] | ||
| + | [[Category: Nishimura, M]] | ||
| + | [[Category: Ohkubo, T]] | ||
| + | [[Category: Taniguchi, T]] | ||
[[Category: Cell adhesion]] | [[Category: Cell adhesion]] | ||
[[Category: Colonization]] | [[Category: Colonization]] | ||
[[Category: Type iv pilin]] | [[Category: Type iv pilin]] | ||
Current revision
Crystal Structure Analysis of the CofA
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