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3aqn

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{{STRUCTURE_3aqn| PDB=3aqn | SCENE= }}
 
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===Complex structure of bacterial protein (apo form II)===
 
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{{ABSTRACT_PUBMED_21300291}}
 
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==About this Structure==
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==Complex structure of bacterial protein (apo form II)==
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[[3aqn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_dh1 Escherichia coli dh1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQN OCA].
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<StructureSection load='3aqn' size='340' side='right'caption='[[3aqn]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3aqn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_DH1 Escherichia coli DH1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AQN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aqn OCA], [https://pdbe.org/3aqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aqn RCSB], [https://www.ebi.ac.uk/pdbsum/3aqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aqn ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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PolyA polymerase (PAP) adds a polyA tail onto the 3'-end of RNAs without a nucleic acid template, using adenosine-5'-triphosphate (ATP) as a substrate. The mechanism for the substrate selection by eubacterial PAP remains obscure. Structural and biochemical studies of Escherichia coli PAP (EcPAP) revealed that the shape and size of the nucleobase-interacting pocket of EcPAP are maintained by an intra-molecular hydrogen-network, making it suitable for the accommodation of only ATP, using a single amino acid, Arg(197). The pocket structure is sustained by interactions between the catalytic domain and the RNA-binding domain. EcPAP has a flexible basic C-terminal region that contributes to optimal RNA translocation for processive adenosine 5'-monophosphate (AMP) incorporations onto the 3'-end of RNAs. A comparison of the EcPAP structure with those of other template-independent RNA polymerases suggests that structural changes of domain(s) outside the conserved catalytic core domain altered the substrate specificities of the template-independent RNA polymerases.
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==See Also==
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Mechanism for the alteration of the substrate specificities of template-independent RNA polymerases.,Toh Y, Takeshita D, Nagaike T, Numata T, Tomita K Structure. 2011 Feb 9;19(2):232-43. PMID:21300291<ref>PMID:21300291</ref>
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*[[Poly(A) Polymerase|Poly(A) Polymerase]]
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:021300291</ref><references group="xtra"/><references/>
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</div>
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[[Category: Escherichia coli dh1]]
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<div class="pdbe-citations 3aqn" style="background-color:#fffaf0;"></div>
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[[Category: Polynucleotide adenylyltransferase]]
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[[Category: Takeshita, D.]]
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==See Also==
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[[Category: Toh, Y.]]
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*[[Poly(A) polymerase 3D structures|Poly(A) polymerase 3D structures]]
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[[Category: Tomita , K.]]
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== References ==
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[[Category: A-phosphorylation]]
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<references/>
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[[Category: Atp binding]]
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__TOC__
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[[Category: Atp-binding]]
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</StructureSection>
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[[Category: Nucleotide-binding]]
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[[Category: Escherichia coli DH1]]
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[[Category: Nucleotidyltransferase]]
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[[Category: Large Structures]]
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[[Category: Rna-binding]]
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[[Category: Takeshita D]]
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[[Category: Transferase]]
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[[Category: Toh Y]]
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[[Category: Transferase/rna]]
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[[Category: Tomita K]]

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Complex structure of bacterial protein (apo form II)

PDB ID 3aqn

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