3vr1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (12:33, 8 November 2023) (edit) (undo)
 
(3 intermediate revisions not shown.)
Line 1: Line 1:
-
{{STRUCTURE_3vr1| PDB=3vr1 | SCENE= }}
 
-
===Crystal structure analysis of the translation factor RF3===
 
-
{{ABSTRACT_PUBMED_22975312}}
 
-
==Function==
+
==Crystal structure analysis of the translation factor RF3==
-
[[http://www.uniprot.org/uniprot/RF3_DESVM RF3_DESVM]] Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP (By similarity).
+
<StructureSection load='3vr1' size='340' side='right'caption='[[3vr1]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3vr1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._'Miyazaki_F' Desulfovibrio vulgaris str. 'Miyazaki F']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VR1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VR1 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G4P:GUANOSINE-5,3-TETRAPHOSPHATE'>G4P</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vr1 OCA], [https://pdbe.org/3vr1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vr1 RCSB], [https://www.ebi.ac.uk/pdbsum/3vr1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vr1 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RF3_DESVM RF3_DESVM] Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP (By similarity).
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The bacterial translational GTPases release factor RF3 promotes translation termination by recycling RF1 or RF2. Here, we present the crystal structures of RF3 complexed with GDP and guanosine 3',5'-(bis)diphosphate (ppGpp) at resolutions of 1.8 and 3.0A, respectively. ppGpp is involved in the so-called "stringent response" of bacteria. ppGpp binds at the same site as GDP, suggesting that GDP and ppGpp are two alternative physiologically relevant ligands of RF3. We also found that ppGpp decelerates the recycling of RF1 by RF3. These lines of evidence suggest that RF3 functions both as a cellular metabolic sensor and as a regulator.
-
==About this Structure==
+
Crystal structure analysis of the translation factor RF3 (release factor 3).,Kihira K, Shimizu Y, Shomura Y, Shibata N, Kitamura M, Nakagawa A, Ueda T, Ochi K, Higuchi Y FEBS Lett. 2012 Oct 19;586(20):3705-9. doi: 10.1016/j.febslet.2012.08.029. Epub, 2012 Sep 6. PMID:22975312<ref>PMID:22975312</ref>
-
[[3vr1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._'miyazaki_f' Desulfovibrio vulgaris str. 'miyazaki f']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VR1 OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<ref group="xtra">PMID:022975312</ref><references group="xtra"/><references/>
+
</div>
-
[[Category: Desulfovibrio vulgaris str. 'miyazaki f']]
+
<div class="pdbe-citations 3vr1" style="background-color:#fffaf0;"></div>
-
[[Category: Higuchi, Y.]]
+
== References ==
-
[[Category: Kihira, K.]]
+
<references/>
-
[[Category: Kitamura, M.]]
+
__TOC__
-
[[Category: Shibata, N.]]
+
</StructureSection>
-
[[Category: Shomura, Y.]]
+
[[Category: Desulfovibrio vulgaris str. 'Miyazaki F']]
-
[[Category: Gtpase]]
+
[[Category: Large Structures]]
-
[[Category: Release factor]]
+
[[Category: Higuchi Y]]
-
[[Category: Translation]]
+
[[Category: Kihira K]]
 +
[[Category: Kitamura M]]
 +
[[Category: Shibata N]]
 +
[[Category: Shomura Y]]

Current revision

Crystal structure analysis of the translation factor RF3

PDB ID 3vr1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3vr1"
Personal tools