4lxf
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4lxf is ON HOLD Authors: Roy, R., Besra, G.S., Futterer, K. Description: Crystal structure of M. tuberculosis TreS) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of M. tuberculosis TreS== | |
| + | <StructureSection load='4lxf' size='340' side='right'caption='[[4lxf]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4lxf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LXF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LXF FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lxf OCA], [https://pdbe.org/4lxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lxf RCSB], [https://www.ebi.ac.uk/pdbsum/4lxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lxf ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Recent evidence established that the cell envelope of Mycobacterium tuberculosis, the bacillus causing tuberculosis (TB), is coated by an alpha-glucan-containing capsule that has been implicated in persistence in a mouse infection model. As one of three known metabolic routes to alpha-glucan in mycobacteria, the cytoplasmic GlgE-pathway converts trehalose to alpha(1 --> 4),alpha(1 --> 6)-linked glucan in 4 steps. Whether individual reaction steps, catalyzed by trehalose synthase TreS, maltokinase Pep2, and glycosyltransferases GlgE and GlgB, occur independently or in a coordinated fashion is not known. Here, we report the crystal structure of M. tuberculosis TreS, and show by small-angle X-ray scattering and analytical ultracentrifugation that TreS forms tetramers in solution. Together with Pep2, TreS forms a hetero-octameric complex, and we demonstrate that complex formation markedly accelerates maltokinase activity of Pep2. Thus, complex formation may act as part of a regulatory mechanism of the GlgE pathway, which overall must avoid accumulation of toxic pathway intermediates, such as maltose-1-phosphate, and optimize the use of scarce nutrients. | ||
| - | + | Synthesis of alpha-Glucan in Mycobacteria Involves a Hetero-octameric Complex of Trehalose Synthase TreS and Maltokinase Pep2.,Roy R, Usha V, Kermani A, Scott DJ, Hyde EI, Besra GS, Alderwick LJ, Futterer K ACS Chem Biol. 2013 Aug 13. PMID:23901909<ref>PMID:23901909</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4lxf" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mycobacterium tuberculosis H37Rv]] | ||
| + | [[Category: Besra GS]] | ||
| + | [[Category: Futterer K]] | ||
| + | [[Category: Roy R]] | ||
Current revision
Crystal structure of M. tuberculosis TreS
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