4g8x

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{{STRUCTURE_4g8x| PDB=4g8x | SCENE= }}
 
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===G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex===
 
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{{ABSTRACT_PUBMED_23178120}}
 
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==Function==
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==G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex==
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[[http://www.uniprot.org/uniprot/SIGA_STAA8 SIGA_STAA8]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.
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<StructureSection load='4g8x' size='340' side='right'caption='[[4g8x]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4g8x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325] and [https://en.wikipedia.org/wiki/Staphylococcus_virus_G1 Staphylococcus virus G1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G8X FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.001&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g8x OCA], [https://pdbe.org/4g8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g8x RCSB], [https://www.ebi.ac.uk/pdbsum/4g8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g8x ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SIGA_STAA8 SIGA_STAA8] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phage G1 gp67 is a 23 kDa protein that binds to the Staphylococcus aureus (Sau) RNA polymerase (RNAP) sigma(A) subunit and blocks cell growth by inhibiting transcription. We show that gp67 has little to no effect on transcription from most promoters but is a potent inhibitor of ribosomal RNA transcription. A 2.0-A-resolution crystal structure of the complex between gp67 and Sau sigma(A) domain 4 (sigma(A)(4)) explains how gp67 joins the RNAP promoter complex through sigma(A)(4) without significantly affecting sigma(A)(4) function. Our results indicate that gp67 forms a complex with RNAP at most, if not all, sigma(A)-dependent promoters, but selectively inhibits promoters that depend on an interaction between upstream DNA and the RNAP alpha-subunit C-terminal domain (alphaCTD). Thus, we reveal a promoter-specific transcription inhibition mechanism by which gp67 interacts with the RNAP promoter complex through one subunit (sigma(A)), and selectively affects the function of another subunit (alphaCTD) depending on promoter usage.
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==About this Structure==
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Promoter-specific transcription inhibition in Staphylococcus aureus by a phage protein.,Osmundson J, Montero-Diez C, Westblade LF, Hochschild A, Darst SA Cell. 2012 Nov 21;151(5):1005-16. doi: 10.1016/j.cell.2012.10.034. PMID:23178120<ref>PMID:23178120</ref>
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[[4g8x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_nctc_8325 Staphylococcus aureus subsp. aureus nctc 8325] and [http://en.wikipedia.org/wiki/Staphylococcus_phage_g1 Staphylococcus phage g1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G8X OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023178120</ref><references group="xtra"/><references/>
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</div>
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[[Category: Staphylococcus aureus subsp. aureus nctc 8325]]
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<div class="pdbe-citations 4g8x" style="background-color:#fffaf0;"></div>
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[[Category: Staphylococcus phage g1]]
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[[Category: Darst, S A.]]
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==See Also==
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[[Category: Hochschild, A.]]
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*[[Sigma factor 3D structures|Sigma factor 3D structures]]
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[[Category: Montero-Diaz, C M.]]
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== References ==
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[[Category: Osmundson, J S.]]
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<references/>
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[[Category: Dna binding protein]]
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__TOC__
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[[Category: Rnap binding protein]]
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
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[[Category: Staphylococcus virus G1]]
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[[Category: Darst SA]]
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[[Category: Hochschild A]]
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[[Category: Montero-Diaz CM]]
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[[Category: Osmundson JS]]

Current revision

G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex

PDB ID 4g8x

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