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3w0p

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{{STRUCTURE_3w0p| PDB=3w0p | SCENE= }}
 
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===Crystal structure of a thermostable mutant of aminoglycoside phosphotransferase APH(4)-Ia (D198A), ternary complex with ADP and hygromycin B===
 
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{{ABSTRACT_PUBMED_23747390}}
 
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==Function==
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==Crystal structure of a thermostable mutant of aminoglycoside phosphotransferase APH(4)-Ia (D198A), ternary complex with ADP and hygromycin B==
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[[http://www.uniprot.org/uniprot/KHYB_ECOLX KHYB_ECOLX]] The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation. Only phosphorylates hygromycin and closely related compounds such as demethyl analogs and destomycin.
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<StructureSection load='3w0p' size='340' side='right'caption='[[3w0p]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[3w0p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W0P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3W0P FirstGlance]. <br>
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[[3w0p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W0P OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=HY0:HYGROMYCIN+B+VARIANT'>HY0</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3w0p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w0p OCA], [https://pdbe.org/3w0p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3w0p RCSB], [https://www.ebi.ac.uk/pdbsum/3w0p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3w0p ProSAT]</span></td></tr>
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<ref group="xtra">PMID:023747390</ref><references group="xtra"/><references/>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KHYB_ECOLX KHYB_ECOLX] The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation. Only phosphorylates hygromycin and closely related compounds such as demethyl analogs and destomycin.
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Hygromycin B 4-O-kinase]]
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[[Category: Large Structures]]
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[[Category: Fukano, K.]]
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[[Category: Fukano K]]
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[[Category: Hoshino, T.]]
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[[Category: Hoshino T]]
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[[Category: Iino, D.]]
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[[Category: Iino D]]
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[[Category: Nakamura, A.]]
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[[Category: Nakamura A]]
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[[Category: Ohsawa, K.]]
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[[Category: Ohsawa K]]
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[[Category: Sasaki, Y.]]
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[[Category: Sasaki Y]]
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[[Category: Takakura, Y.]]
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[[Category: Takakura Y]]
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[[Category: Yajima, S.]]
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[[Category: Yajima S]]
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[[Category: Phosphotransferase]]
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[[Category: Transferase-antibiotic complex]]
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Current revision

Crystal structure of a thermostable mutant of aminoglycoside phosphotransferase APH(4)-Ia (D198A), ternary complex with ADP and hygromycin B

PDB ID 3w0p

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