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2jm3
From Proteopedia
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| - | [[Image:2jm3.jpg|left|200px]]<br /><applet load="2jm3" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2jm3" /> | ||
| - | '''Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)'''<br /> | ||
| - | == | + | ==Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)== |
| + | <StructureSection load='2jm3' size='340' side='right'caption='[[2jm3]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2jm3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JM3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JM3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jm3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jm3 OCA], [https://pdbe.org/2jm3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jm3 RCSB], [https://www.ebi.ac.uk/pdbsum/2jm3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jm3 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CTBP1_CAEEL CTBP1_CAEEL] Binds DNA and represses gene expression. Plays a role in regulation of life span, possibly by regulating transcription of genes important for lipid metabolism.<ref>PMID:18005989</ref> <ref>PMID:19164523</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
The THAP (Thanatos-associated protein) domain is a recently discovered zinc-binding domain found in proteins involved in transcriptional regulation, cell-cycle control, apoptosis and chromatin modification. It contains a single zinc atom ligated by cysteine and histidine residues within a Cys-X(2-4)-Cys-X(35-53)-Cys-X(2)-His consensus. We have determined the NMR solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP) and show that it adopts a fold containing a treble clef motif, bearing similarity to the zinc finger-associated domain (ZAD) from Drosophila Grauzone. The CtBP THAP domain contains a large, positively charged surface patch and we demonstrate that this domain can bind to double-stranded DNA in an electrophoretic mobility-shift assay. These data, together with existing reports, indicate that THAP domains might exhibit a functional diversity similar to that observed for classical and GATA-type zinc fingers. | The THAP (Thanatos-associated protein) domain is a recently discovered zinc-binding domain found in proteins involved in transcriptional regulation, cell-cycle control, apoptosis and chromatin modification. It contains a single zinc atom ligated by cysteine and histidine residues within a Cys-X(2-4)-Cys-X(35-53)-Cys-X(2)-His consensus. We have determined the NMR solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP) and show that it adopts a fold containing a treble clef motif, bearing similarity to the zinc finger-associated domain (ZAD) from Drosophila Grauzone. The CtBP THAP domain contains a large, positively charged surface patch and we demonstrate that this domain can bind to double-stranded DNA in an electrophoretic mobility-shift assay. These data, together with existing reports, indicate that THAP domains might exhibit a functional diversity similar to that observed for classical and GATA-type zinc fingers. | ||
| - | + | Solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP).,Liew CK, Crossley M, Mackay JP, Nicholas HR J Mol Biol. 2007 Feb 16;366(2):382-90. Epub 2006 Nov 18. PMID:17174978<ref>PMID:17174978</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 2jm3" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Caenorhabditis elegans]] | [[Category: Caenorhabditis elegans]] | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Crossley | + | [[Category: Crossley M]] |
| - | [[Category: Liew | + | [[Category: Liew CK]] |
| - | [[Category: Mackay | + | [[Category: Mackay JP]] |
| - | [[Category: Nicholas | + | [[Category: Nicholas HR]] |
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Current revision
Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)
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