4i60

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{{STRUCTURE_4i60| PDB=4i60 | SCENE= }}
 
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===Crystal structure of avidin - biotinylruthenocene complex===
 
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{{ABSTRACT_PUBMED_23603015}}
 
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==Function==
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==Crystal structure of avidin - biotinylruthenocene complex==
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[[http://www.uniprot.org/uniprot/AVID_CHICK AVID_CHICK]] The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
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<StructureSection load='4i60' size='340' side='right'caption='[[4i60]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4i60]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4I60 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B1R:[(1,2,3,4,5-ETA)-CYCLOPENTADIENYL][(1,2,3,4,5-ETA)-{5-[(3AS,4S,6AR)-2-OXOHEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL]PENTANOYL}CYCLOPENTADIENYL]RUTHENIUM'>B1R</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4i60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i60 OCA], [https://pdbe.org/4i60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4i60 RCSB], [https://www.ebi.ac.uk/pdbsum/4i60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4i60 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AVID_CHICK AVID_CHICK] The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of avidin, a protein from hen egg white, was determined in the form of a complex with biotinylruthenocene. This biotin-derived organometallic ligand is a potential anticancer agent for targeted therapy based upon avidin-biotin technology. Isothermal titration calorimetry experiments, involving avidin complexes with biotin (vitamin H or B7) derivatives, show differences in their affinity to the protein in comparison to its avidin-biotin complex, the strongest known biochemical interaction in Nature. The crystal structure of the first complex of avidin with biotinylruthenocene, determined at 2.5A resolution (PDB: 4I60), shows unique interactions of the ruthenocene moiety with avidin. Biotin derivatives exhibit weaker affinity to avidin then biotin, which allows their wider use in biotechnology. The specific properties of biotinylruthenocene and the knowledge of its interactions with avidin may be useful in biochemical, medical, and nanotechnological applications.
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==About this Structure==
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Structural investigation of the interactions of biotinylruthenocene with avidin.,Strzelczyk P, Bujacz A, Plazuk D, Zakrzewski J, Bujacz G Chem Biol Interact. 2013 Jun 25;204(1):6-12. doi: 10.1016/j.cbi.2013.04.005. Epub, 2013 Apr 18. PMID:23603015<ref>PMID:23603015</ref>
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[[4i60]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I60 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023603015</ref><references group="xtra"/><references/>
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</div>
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<div class="pdbe-citations 4i60" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Avidin 3D structures|Avidin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Bujacz, A.]]
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[[Category: Large Structures]]
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[[Category: Bujacz, G.]]
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[[Category: Bujacz A]]
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[[Category: Strzelczyk, P.]]
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[[Category: Bujacz G]]
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[[Category: Beta barrel]]
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[[Category: Strzelczyk P]]
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[[Category: Biotin-binding protein]]
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[[Category: Biotinylruthenocene]]
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[[Category: Glycoprotein]]
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[[Category: Hen egg white]]
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Current revision

Crystal structure of avidin - biotinylruthenocene complex

PDB ID 4i60

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