4bhu

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{{STRUCTURE_4bhu| PDB=4bhu | SCENE= }}
 
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===Crystal structure of BslA - A bacterial hydrophobin===
 
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{{ABSTRACT_PUBMED_23904481}}
 
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==Function==
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==Crystal structure of BslA - A bacterial hydrophobin==
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[[http://www.uniprot.org/uniprot/YUAB_BACSU YUAB_BACSU]] Required for complex colony architecture.
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<StructureSection load='4bhu' size='340' side='right'caption='[[4bhu]], [[Resolution|resolution]] 1.91&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4bhu]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_NCIB_3610_=_ATCC_6051_=_DSM_10 Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BHU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BHU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.91&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bhu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bhu OCA], [https://pdbe.org/4bhu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bhu RCSB], [https://www.ebi.ac.uk/pdbsum/4bhu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bhu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BSLA_BACSU BSLA_BACSU] Involved in biofilm formation (PubMed:18978066, PubMed:21742882). Self-polymerizes and forms a layer on the surface of biofilms that confers hydrophobicity to the biofilm (PubMed:22571672, PubMed:23904481). The layer is stable and capable of resistance to high mechanical force compression (PubMed:28701036). Required for complex colony architecture (PubMed:18978066). May function synergistically with exopolysaccharides and TasA amyloid fibers to facilitate the assembly of the biofilm matrix (PubMed:21742882).<ref>PMID:18978066</ref> <ref>PMID:21742882</ref> <ref>PMID:22571672</ref> <ref>PMID:23904481</ref> <ref>PMID:28701036</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Biofilms represent the predominant mode of microbial growth in the natural environment. Bacillus subtilis is a ubiquitous Gram-positive soil bacterium that functions as an effective plant growth-promoting agent. The biofilm matrix is composed of an exopolysaccharide and an amyloid fiber-forming protein, TasA, and assembles with the aid of a small secreted protein, BslA. Here we show that natively synthesized and secreted BslA forms surface layers around the biofilm. Biophysical analysis demonstrates that BslA can self-assemble at interfaces, forming an elastic film. Molecular function is revealed from analysis of the crystal structure of BslA, which consists of an Ig-type fold with the addition of an unusual, extremely hydrophobic "cap" region. A combination of in vivo biofilm formation and in vitro biophysical analysis demonstrates that the central hydrophobic residues of the cap are essential to allow a hydrophobic, nonwetting biofilm to form as they control the surface activity of the BslA protein. The hydrophobic cap exhibits physiochemical properties remarkably similar to the hydrophobic surface found in fungal hydrophobins; thus, BslA is a structurally defined bacterial hydrophobin. We suggest that biofilms formed by other species of bacteria may have evolved similar mechanisms to provide protection to the resident bacterial community.
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==About this Structure==
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BslA is a self-assembling bacterial hydrophobin that coats the Bacillus subtilis biofilm.,Hobley L, Ostrowski A, Rao FV, Bromley KM, Porter M, Prescott AR, Macphee CE, van Aalten DM, Stanley-Wall NR Proc Natl Acad Sci U S A. 2013 Jul 31. PMID:23904481<ref>PMID:23904481</ref>
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[[4bhu]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._ncib_3610 Bacillus subtilis subsp. subtilis str. ncib 3610]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BHU OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023904481</ref><references group="xtra"/><references/>
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</div>
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[[Category: Bacillus subtilis subsp. subtilis str. ncib 3610]]
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<div class="pdbe-citations 4bhu" style="background-color:#fffaf0;"></div>
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[[Category: Aalten, D M.F van.]]
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== References ==
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[[Category: Bromley, K M.]]
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<references/>
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[[Category: Hobley, L.]]
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__TOC__
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[[Category: MacPhee, C E.]]
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</StructureSection>
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[[Category: Ostrowski, A.]]
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[[Category: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10]]
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[[Category: Porter, M.]]
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[[Category: Large Structures]]
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[[Category: Prescott, A R.]]
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[[Category: Bromley KM]]
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[[Category: Rao, F V.]]
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[[Category: Hobley L]]
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[[Category: Stanley-Wall, N R.]]
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[[Category: MacPhee CE]]
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[[Category: Swedlow, J R.]]
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[[Category: Ostrowski A]]
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[[Category: Biofilm]]
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[[Category: Porter M]]
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[[Category: Structural protein]]
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[[Category: Prescott AR]]
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[[Category: Rao FV]]
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[[Category: Stanley-Wall NR]]
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[[Category: Swedlow JR]]
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[[Category: Van Aalten DMF]]

Current revision

Crystal structure of BslA - A bacterial hydrophobin

PDB ID 4bhu

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