2lkf

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[[Image:2lkf.jpg|left|200px]]<br /><applet load="2lkf" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2lkf, resolution 2.5&Aring;" />
 
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'''LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS'''<br />
 
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==Overview==
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==LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS==
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Staphylococcal LukF, LukS, HgammaII, and alpha-hemolysin are self-assembling, channel-forming proteins related in sequence and function. In the alpha-hemolysin heptamer, the channel-forming beta-strands and the amino latch make long excursions from the protomer core. Here we report the crystal structure of the water soluble form of LukF. In the LukF structure the channel-forming region folds into an amphipathic, three-strand beta-sheet and the amino latch forms a beta-strand extending a central beta-sheet. The LukF structure illustrates how a channel-forming toxin masks protein-protein and protein-membrane interfaces prior to cell binding and assembly, and together with the alpha-hemolysin heptamer structure, they define the end points on the pathway of toxin assembly.
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<StructureSection load='2lkf' size='340' side='right'caption='[[2lkf]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2lkf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LKF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LKF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lkf OCA], [https://pdbe.org/2lkf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lkf RCSB], [https://www.ebi.ac.uk/pdbsum/2lkf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lkf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HLGB_STAAU HLGB_STAAU] Toxin that seems to act by forming pores in the membrane of the cell. Has a hemolytic and a leucotoxic activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lk/2lkf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2lkf ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2LKF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LKF OCA].
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*[[Pore forming toxin%2C α-hemolysin|Pore forming toxin%2C α-hemolysin]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of staphylococcal LukF delineates conformational changes accompanying formation of a transmembrane channel., Olson R, Nariya H, Yokota K, Kamio Y, Gouaux E, Nat Struct Biol. 1999 Feb;6(2):134-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10048924 10048924]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Gouaux, J E.]]
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[[Category: Gouaux JE]]
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[[Category: Kamio, Y.]]
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[[Category: Kamio Y]]
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[[Category: Nariya, H.]]
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[[Category: Nariya H]]
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[[Category: Olson, R.]]
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[[Category: Olson R]]
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[[Category: Yokota, K.]]
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[[Category: Yokota K]]
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[[Category: hemolysin]]
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[[Category: leukotoxin]]
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[[Category: pore-forming toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:07:15 2008''
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Current revision

LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS

PDB ID 2lkf

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