4e2i

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{{STRUCTURE_4e2i| PDB=4e2i | SCENE= }}
 
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===The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase===
 
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{{ABSTRACT_PUBMED_22700977}}
 
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==Function==
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==The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase==
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[[http://www.uniprot.org/uniprot/DPOA2_HUMAN DPOA2_HUMAN]] May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity).
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<StructureSection load='4e2i' size='340' side='right'caption='[[4e2i]], [[Resolution|resolution]] 5.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[4e2i]] is a 23 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Macaca_mulatta_polyomavirus_1 Macaca mulatta polyomavirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E2I FirstGlance]. <br>
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[[4e2i]] is a 23 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2I OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2i OCA], [https://pdbe.org/4e2i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e2i RCSB], [https://www.ebi.ac.uk/pdbsum/4e2i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LT_SV40 LT_SV40] Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA, abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription. May inactivate the growth-suppressing properties of the E3 ubiquitin ligase CUL7.<ref>PMID:8647434</ref> <ref>PMID:9632777</ref> <ref>PMID:9488456</ref> <ref>PMID:15680424</ref> <ref>PMID:15611062</ref> <ref>PMID:17341466</ref> <ref>PMID:18922873</ref> Isoform 17kT antigen targets host RBL2 for degradation and promotes cell proliferation. Transactivates host cyclin A promoter through its J domain.<ref>PMID:8647434</ref> <ref>PMID:9632777</ref> <ref>PMID:9488456</ref> <ref>PMID:15680424</ref> <ref>PMID:15611062</ref> <ref>PMID:17341466</ref> <ref>PMID:18922873</ref>
==See Also==
==See Also==
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*[[DNA polymerase|DNA polymerase]]
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*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
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*[[Large T Antigen|Large T Antigen]]
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==Reference==
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== References ==
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<ref group="xtra">PMID:022700977</ref><references group="xtra"/><references/>
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Simian virus 40]]
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[[Category: Large Structures]]
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[[Category: Arnett, D R.]]
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[[Category: Macaca mulatta polyomavirus 1]]
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[[Category: Brewster, A.]]
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[[Category: Arnett DR]]
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[[Category: Chen, X S.]]
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[[Category: Brewster A]]
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[[Category: Fanning, E.]]
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[[Category: Chen XS]]
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[[Category: Gai, D.]]
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[[Category: Fanning E]]
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[[Category: Sowd, G A.]]
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[[Category: Gai D]]
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[[Category: Vila, S.]]
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[[Category: Sowd GA]]
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[[Category: Xie, C L.]]
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[[Category: Vila S]]
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[[Category: Yu, X.]]
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[[Category: Xie CL]]
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[[Category: Zhou, B.]]
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[[Category: Yu X]]
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[[Category: Hydrolase-dna binding complex]]
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[[Category: Zhou B]]
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[[Category: Hydrolase-dna binding protein complex]]
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[[Category: Replication initiation]]
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Current revision

The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase

PDB ID 4e2i

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