4kyp

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'''Unreleased structure'''
 
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The entry 4kyp is ON HOLD until Paper Publication
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==Beta-Scorpion Toxin folded in the periplasm of E.coli==
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<StructureSection load='4kyp' size='340' side='right'caption='[[4kyp]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4kyp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Hottentotta_judaicus Hottentotta judaicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KYP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KYP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kyp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kyp OCA], [https://pdbe.org/4kyp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kyp RCSB], [https://www.ebi.ac.uk/pdbsum/4kyp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kyp ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BACKGROUND: Animal neurotoxin peptides are valuable probes for investigating ion channel structure/function relationships and represent lead compounds for novel therapeutics and insecticides. However, misfolding and aggregation are common outcomes when toxins containing multiple disulfides are expressed in bacteria. METHODS: The beta-scorpion peptide toxin Bj-xtrIT from Hottentotta judaica and four chaperone enzymes (DsbA, DsbC, SurA and FkpA) were co-secreted into the oxidizing environment of the Escherichia coli periplasm. Expressed Bj-xtrIT was purified and analyzed by HPLC and FPLC chromatography. Its thermostability was assessed using synchrotron radiation circular dichroism spectroscopy and its crystal structure was determined. RESULTS: Western blot analysis showed that robust expression was only achieved when cells co-expressed the chaperones. The purified samples were homogenous and monodisperse and the protein was thermostable. The crystal structure of the recombinant toxin confirmed that it adopts the native disulfide connectivity and fold. CONCLUSIONS: The chaperones enabled correct folding of the four-disulfide-bridged Bj-xtrIT toxin. There was no apparent sub-population of misfolded Bj-xtrIT, which attests to the effectiveness of this expression method. GENERAL SIGNIFICANCE: We report the first example of a disulfide-linked scorpion toxin natively folded during bacterial expression. This method eliminates downstream processing steps such as oxidative refolding or cleavage of a fusion-carrier and therefore enables efficient production of insecticidal Bj-xtrIT. Periplasmic chaperone activity may produce native folding of other extensively disulfide-reticulated proteins including animal neurotoxins. This work is therefore relevant to venomics and studies of a wide range of channels and receptors.
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Authors: O'Reilly, A.O, Cole, A.R., Lopes, J.L., Lampert, A., Wallace, B.A.
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Chaperone-mediated native folding of a beta-scorpion toxin in the periplasm of Escherichia coli.,O'Reilly AO, Cole AR, Lopes JL, Lampert A, Wallace BA Biochim Biophys Acta. 2014 Jan;1840(1):10-5. doi: 10.1016/j.bbagen.2013.08.021., Epub 2013 Aug 30. PMID:23999087<ref>PMID:23999087</ref>
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Description: Beta-Scorpion Toxin folded in the periplasm of E.coli
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4kyp" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Hottentotta judaicus]]
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[[Category: Large Structures]]
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[[Category: Cole AR]]
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[[Category: Lampert A]]
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[[Category: Lopes JL]]
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[[Category: O'Reilly AO]]
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[[Category: Wallace BA]]

Current revision

Beta-Scorpion Toxin folded in the periplasm of E.coli

PDB ID 4kyp

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