4kx9
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| - | {{STRUCTURE_4kx9| PDB=4kx9 | SCENE= }} | ||
| - | ===Crystal structure of human aminopeptidase A complexed with arginine=== | ||
| - | {{ABSTRACT_PUBMED_23888046}} | ||
| - | == | + | ==Crystal structure of human aminopeptidase A complexed with arginine== |
| - | [[http://www.uniprot.org/uniprot/AMPE_HUMAN AMPE_HUMAN | + | <StructureSection load='4kx9' size='340' side='right'caption='[[4kx9]], [[Resolution|resolution]] 2.25Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4kx9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KX9 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kx9 OCA], [https://pdbe.org/4kx9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kx9 RCSB], [https://www.ebi.ac.uk/pdbsum/4kx9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kx9 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/AMPE_HUMAN AMPE_HUMAN] Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Hypertension is regulated through both the central and systemic renin-angiotensin systems. In the central renin-angiotensin system, zinc-dependent aminopeptidase A (APA) up-regulates blood pressure by specifically cleaving the N-terminal aspartate, but not the adjacent arginine, from angiotensin II, a process facilitated by calcium. Here, we determined the crystal structures of human APA and its complexes with different ligands and identified a calcium-binding site in the S1 pocket of APA. Without calcium, the S1 pocket can bind both acidic and basic residues through formation of salt bridges with the charged side chains. In the presence of calcium, the binding of acidic residues is enhanced as they ligate the cation, whereas the binding of basic residues is no longer favorable due to charge repulsion. Of the peptidomimetic inhibitors of APA, amastatin has higher potency than bestatin by fitting better in the S1 pocket and interacting additionally with the S3' subsite. These results explain the calcium-modulated substrate specificity of APA in central hypertension regulation and can guide the design and development of brain-targeting antihypertensive APA inhibitors. | ||
| - | + | Structural insights into central hypertension regulation by human aminopeptidase a.,Yang Y, Liu C, Lin YL, Li F J Biol Chem. 2013 Aug 30;288(35):25638-45. doi: 10.1074/jbc.M113.494955. Epub, 2013 Jul 25. PMID:23888046<ref>PMID:23888046</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | <div class="pdbe-citations 4kx9" style="background-color:#fffaf0;"></div> | ||
| - | == | + | ==See Also== |
| - | + | *[[Aminopeptidase 3D structures|Aminopeptidase 3D structures]] | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Li | + | [[Category: Large Structures]] |
| - | [[Category: Lin | + | [[Category: Li F]] |
| - | [[Category: Liu | + | [[Category: Lin YY]] |
| - | [[Category: Yang | + | [[Category: Liu C]] |
| - | + | [[Category: Yang Y]] | |
| - | + | ||
Current revision
Crystal structure of human aminopeptidase A complexed with arginine
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Categories: Homo sapiens | Large Structures | Li F | Lin YY | Liu C | Yang Y
