2o42

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[[Image:2o42.jpg|left|200px]]<br /><applet load="2o42" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2o42, resolution 2.91&Aring;" />
 
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'''Crystal Structure of M11L, Bcl-2 homolog from myxoma virus'''<br />
 
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==Overview==
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==Crystal Structure of M11L, Bcl-2 homolog from myxoma virus==
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<StructureSection load='2o42' size='340' side='right'caption='[[2o42]], [[Resolution|resolution]] 2.91&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2o42]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxoma_virus Myxoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O42 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O42 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.91&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o42 OCA], [https://pdbe.org/2o42 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o42 RCSB], [https://www.ebi.ac.uk/pdbsum/2o42 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o42 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q85295_9POXV Q85295_9POXV]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Apoptosis of virally infected cells is an innate host mechanism used to prevent viral spread. However, viruses have evolved a number of proteins that function to modulate the apoptotic cascades and thereby favor productive viral replication. One such antiapoptotic protein, myxoma virus M11L, has been shown to inhibit mitochondrial-dependent apoptosis by binding to and blocking the two executioner proteins Bak and Bax. Since M11L has no obvious sequence homology with Bcl-2 or Bcl-x(L), the normal cellular inhibitors for Bak and Bax, and the structure of M11L has not been solved, the mode of binding to Bak and Bax is not known. In order to understand how M11L functions, the crystal structure of M11L was solved to 2.91 A. Despite the lack of sequence similarity, M11L is a structural homolog of Bcl-2. Studies using a peptide derived from Bak indicate that M11L binds to Bak with a similar affinity (4.9 +/- 0.3 microM) to the published binding affinities of Bcl-2 and Bcl-x(L) to the same peptide (12.7 microM and 0.5 microM, respectively), indicating that M11L inhibits apoptosis by mimicking and competing with host proteins for the binding of Bak and Bax. The structure provides important insight into how myxoma virus and other poxviruses facilitate viral dissemination by inhibiting mitochondrial dependent apoptosis.
Apoptosis of virally infected cells is an innate host mechanism used to prevent viral spread. However, viruses have evolved a number of proteins that function to modulate the apoptotic cascades and thereby favor productive viral replication. One such antiapoptotic protein, myxoma virus M11L, has been shown to inhibit mitochondrial-dependent apoptosis by binding to and blocking the two executioner proteins Bak and Bax. Since M11L has no obvious sequence homology with Bcl-2 or Bcl-x(L), the normal cellular inhibitors for Bak and Bax, and the structure of M11L has not been solved, the mode of binding to Bak and Bax is not known. In order to understand how M11L functions, the crystal structure of M11L was solved to 2.91 A. Despite the lack of sequence similarity, M11L is a structural homolog of Bcl-2. Studies using a peptide derived from Bak indicate that M11L binds to Bak with a similar affinity (4.9 +/- 0.3 microM) to the published binding affinities of Bcl-2 and Bcl-x(L) to the same peptide (12.7 microM and 0.5 microM, respectively), indicating that M11L inhibits apoptosis by mimicking and competing with host proteins for the binding of Bak and Bax. The structure provides important insight into how myxoma virus and other poxviruses facilitate viral dissemination by inhibiting mitochondrial dependent apoptosis.
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==About this Structure==
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Structure of M11L: A myxoma virus structural homolog of the apoptosis inhibitor, Bcl-2.,Douglas AE, Corbett KD, Berger JM, McFadden G, Handel TM Protein Sci. 2007 Apr;16(4):695-703. PMID:17384234<ref>PMID:17384234</ref>
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2O42 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Myxoma_virus Myxoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O42 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of M11L: A myxoma virus structural homolog of the apoptosis inhibitor, Bcl-2., Douglas AE, Corbett KD, Berger JM, McFadden G, Handel TM, Protein Sci. 2007 Apr;16(4):695-703. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17384234 17384234]
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</div>
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[[Category: Myxoma virus]]
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<div class="pdbe-citations 2o42" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Douglas, A E.]]
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[[Category: apoptosis inhibitor]]
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[[Category: bcl-2 homolog]]
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[[Category: poxvirus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:14:17 2008''
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==See Also==
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*[[B-cell lymphoma proteins 3D structures|B-cell lymphoma proteins 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Myxoma virus]]
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[[Category: Douglas AE]]

Current revision

Crystal Structure of M11L, Bcl-2 homolog from myxoma virus

PDB ID 2o42

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