2ob4

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[[Image:2ob4.gif|left|200px]]<br /><applet load="2ob4" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ob4, resolution 2.40&Aring;" />
 
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'''Human Ubiquitin-Conjugating Enzyme CDC34'''<br />
 
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==About this Structure==
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==Human Ubiquitin-Conjugating Enzyme CDC34==
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2OB4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OB4 OCA].
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<StructureSection load='2ob4' size='340' side='right'caption='[[2ob4]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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[[Category: Homo sapiens]]
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== Structural highlights ==
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[[Category: Single protein]]
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<table><tr><td colspan='2'>[[2ob4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OB4 FirstGlance]. <br>
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[[Category: Ubiquitin--protein ligase]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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[[Category: Arrowsmith, C H.]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ob4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ob4 OCA], [https://pdbe.org/2ob4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ob4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ob4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ob4 ProSAT]</span></td></tr>
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[[Category: Avvakumov, G V.]]
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</table>
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[[Category: Bochkarev, A.]]
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== Function ==
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[[Category: Dhe-Paganon, S.]]
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[https://www.uniprot.org/uniprot/UB2R1_HUMAN UB2R1_HUMAN] Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.<ref>PMID:10329681</ref> <ref>PMID:10373550</ref> <ref>PMID:10871850</ref> <ref>PMID:11675391</ref> <ref>PMID:12037680</ref> <ref>PMID:15652359</ref> <ref>PMID:17461777</ref> <ref>PMID:17698585</ref> <ref>PMID:19945379</ref> <ref>PMID:19126550</ref> <ref>PMID:20061386</ref> <ref>PMID:20347421</ref>
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[[Category: Edwards, A M.]]
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== Evolutionary Conservation ==
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[[Category: Mackenzie, F.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Neculai, D.]]
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Check<jmol>
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[[Category: SGC, Structural Genomics Consortium.]]
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<jmolCheckbox>
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[[Category: Sicheri, F.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ob/2ob4_consurf.spt"</scriptWhenChecked>
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[[Category: Sundstrom, M.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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[[Category: Walker, J R.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Weigelt, J.]]
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</jmolCheckbox>
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[[Category: Xue, S.]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ob4 ConSurf].
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[[Category: ligase; ubl conjugation pathway; structural genomics consortium]]
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<div style="clear:both"></div>
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[[Category: sgc]]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Here we describe a systematic structure-function analysis of the human ubiquitin (Ub) E2 conjugating proteins, consisting of the determination of 15 new high-resolution three-dimensional structures of E2 catalytic domains, and autoubiquitylation assays for 26 Ub-loading E2s screened against a panel of nine different HECT (homologous to E6-AP carboxyl terminus) E3 ligase domains. Integration of our structural and biochemical data revealed several E2 surface properties associated with Ub chain building activity; (1) net positive or neutral E2 charge, (2) an "acidic trough" located near the catalytic Cys, surrounded by an extensive basic region, and (3) similarity to the previously described HECT binding signature in UBE2L3 (UbcH7). Mass spectrometry was used to characterize the autoubiquitylation products of a number of functional E2-HECT pairs, and demonstrated that HECT domains from different subfamilies catalyze the formation of very different types of Ub chains, largely independent of the E2 in the reaction. Our data set represents the first comprehensive analysis of E2-HECT E3 interactions, and thus provides a framework for better understanding the molecular mechanisms of ubiquitylation.
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:16:30 2008''
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A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen.,Sheng Y, Hong JH, Doherty R, Srikumar T, Shloush J, Avvakumov GV, Walker JR, Xue S, Neculai D, Wan JW, Kim SK, Arrowsmith CH, Raught B, Dhe-Paganon S Mol Cell Proteomics. 2012 Aug;11(8):329-41. Epub 2012 Apr 10. PMID:22496338<ref>PMID:22496338</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2ob4" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Arrowsmith CH]]
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[[Category: Avvakumov GV]]
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[[Category: Bochkarev A]]
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[[Category: Dhe-Paganon S]]
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[[Category: Edwards AM]]
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[[Category: Mackenzie F]]
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[[Category: Neculai D]]
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[[Category: Sicheri F]]
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[[Category: Sundstrom M]]
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[[Category: Walker JR]]
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[[Category: Weigelt J]]
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[[Category: Xue S]]

Current revision

Human Ubiquitin-Conjugating Enzyme CDC34

PDB ID 2ob4

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