4jas

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{{STRUCTURE_4jas| PDB=4jas | SCENE= }}
 
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===Structural basis of a rationally rewired protein-protein interface (HK853mutant A268V, A271G, T275M, V294T and D297E and RR468mutant V13P, L14I, I17M and N21V)===
 
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{{ABSTRACT_PUBMED_23954504}}
 
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==About this Structure==
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==Structural basis of a rationally rewired protein-protein interface (HK853mutant A268V, A271G, T275M, V294T and D297E and RR468mutant V13P, L14I, I17M and N21V)==
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[[4jas]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima_msb8 Thermotoga maritima msb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JAS OCA].
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<StructureSection load='4jas' size='340' side='right'caption='[[4jas]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4jas]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JAS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BFD:ASPARTATE+BERYLLIUM+TRIFLUORIDE'>BFD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jas OCA], [https://pdbe.org/4jas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jas RCSB], [https://www.ebi.ac.uk/pdbsum/4jas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jas ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9WZV7_THEMA Q9WZV7_THEMA]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Two-component signal transduction systems typically involve a sensor histidine kinase that specifically phosphorylates a single, cognate response regulator. This protein-protein interaction relies on molecular recognition via a small set of residues in each protein. To better understand how these residues determine the specificity of kinase-substrate interactions, we rationally rewired the interaction interface of a Thermotoga maritima two-component system, HK853-RR468, to match that found in a different two-component system, Escherichia coli PhoR-PhoB. The rewired proteins interacted robustly with each other, but no longer interacted with the parent proteins. Analysis of the crystal structures of the wild-type and mutant protein complexes and a systematic mutagenesis study reveal how individual mutations contribute to the rewiring of interaction specificity. Our approach and conclusions have implications for studies of other protein-protein interactions and protein evolution and for the design of novel protein interfaces.
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==Reference==
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Structural Basis of a Rationally Rewired Protein-Protein Interface Critical to Bacterial Signaling.,Podgornaia AI, Casino P, Marina A, Laub MT Structure. 2013 Aug 13. pii: S0969-2126(13)00254-2. doi:, 10.1016/j.str.2013.07.005. PMID:23954504<ref>PMID:23954504</ref>
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<ref group="xtra">PMID:023954504</ref><references group="xtra"/><references/>
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[[Category: Histidine kinase]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Thermotoga maritima msb8]]
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</div>
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[[Category: Casino, P.]]
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<div class="pdbe-citations 4jas" style="background-color:#fffaf0;"></div>
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[[Category: Laub, M T.]]
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[[Category: Marina, A.]]
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==See Also==
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[[Category: Podgornaia, A I.]]
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*[[Response regulator 3D structure|Response regulator 3D structure]]
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[[Category: Alpha/beta domain]]
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== References ==
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[[Category: Autophosphorylation]]
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<references/>
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[[Category: Bergerat fold]]
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__TOC__
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[[Category: Dephosphorylation]]
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</StructureSection>
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[[Category: Histidine kinase]]
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[[Category: Large Structures]]
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[[Category: Phosphorylation]]
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[[Category: Thermotoga maritima MSB8]]
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[[Category: Phosphotransferase]]
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[[Category: Casino P]]
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[[Category: Response regulator]]
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[[Category: Laub MT]]
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[[Category: Signal transduction]]
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[[Category: Marina A]]
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[[Category: Transferase-signaling protein complex]]
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[[Category: Podgornaia AI]]

Current revision

Structural basis of a rationally rewired protein-protein interface (HK853mutant A268V, A271G, T275M, V294T and D297E and RR468mutant V13P, L14I, I17M and N21V)

PDB ID 4jas

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