2yjv

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{{STRUCTURE_2yjv| PDB=2yjv | SCENE= }}
 
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===Crystal structure of E. coli regulator of ribonuclease activity A (RraA) bound to fragment of DEAD-box protein RhlB===
 
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{{ABSTRACT_PUBMED_24045937}}
 
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==Function==
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==Crystal structure of E. coli regulator of ribonuclease activity A (RraA) bound to fragment of DEAD-box protein RhlB==
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[[http://www.uniprot.org/uniprot/RRAA_ECOLI RRAA_ECOLI]] Globally modulates RNA abundance by binding to RNase E (Rne) and regulating its endonucleolytic activity. Can modulate Rne action in a substrate-dependent manner by altering the composition of the degradosome. Modulates RNA-binding and helicase activities of the degradosome.<ref>PMID:13678585</ref> <ref>PMID:16725107</ref> <ref>PMID:16771842</ref> <ref>PMID:18510556</ref> <ref>PMID:20106955</ref>
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<StructureSection load='2yjv' size='340' side='right'caption='[[2yjv]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2yjv]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YJV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjv OCA], [https://pdbe.org/2yjv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yjv RCSB], [https://www.ebi.ac.uk/pdbsum/2yjv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yjv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RRAA_ECOLI RRAA_ECOLI] Globally modulates RNA abundance by binding to RNase E (Rne) and regulating its endonucleolytic activity. Can modulate Rne action in a substrate-dependent manner by altering the composition of the degradosome. Modulates RNA-binding and helicase activities of the degradosome.<ref>PMID:13678585</ref> <ref>PMID:16725107</ref> <ref>PMID:16771842</ref> <ref>PMID:18510556</ref> <ref>PMID:20106955</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Members of the DEAD-box family of RNA helicases contribute to virtually every aspect of RNA metabolism, in organisms from all domains of life. Many of these helicases are constituents of multi-component assemblies, and their interactions with partner proteins within the complexes underpin their activities and biological function. In Escherichia coli the DEAD-box helicase RhlB is a component of the multi-enzyme RNA degradosome assembly, and its interaction with the core ribonuclease RNase E boosts the ATP-dependent activity of the helicase (1,2). Earlier studies have identified the regulator of ribonuclease activity A (RraA) as a potential interaction partner of both RNase E and RhlB (3). We present structural and biochemical evidence showing how RraA can bind to, and modulate the activity of RhlB and another E. coli DEAD-box enzyme, SrmB. Crystallographic structures are presented of RraA in complex with a portion of the natively unstructured C-terminal tail of RhlB at 2.8 A resolution, and in complex with the C-terminal RecA-like domain of SrmB at 2.9 A. The models suggest two distinct mechanisms by which RraA might modulate the activity of these and potentially other helicases.
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==About this Structure==
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Potential regulatory interactions of Escherichia coli RraA protein with DEAD-box helicases.,Pietras Z, Hardwick SW, Swiezewski S, Luisi BF J Biol Chem. 2013 Sep 17. PMID:24045937<ref>PMID:24045937</ref>
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[[2yjv]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJV OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024045937</ref><references group="xtra"/><references/>
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</div>
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[[Category: DNA helicase]]
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<div class="pdbe-citations 2yjv" style="background-color:#fffaf0;"></div>
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[[Category: Escherichia coli k-12]]
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== References ==
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[[Category: Hardwick, S W.]]
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<references/>
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[[Category: Luisi, B F.]]
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__TOC__
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[[Category: Pietras, Z.]]
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</StructureSection>
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[[Category: Dead box rna helicase]]
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[[Category: Escherichia coli K-12]]
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[[Category: Hydrolase inhibitor-hydrolase complex]]
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[[Category: Large Structures]]
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[[Category: Hardwick SW]]
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[[Category: Luisi BF]]
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[[Category: Pietras Z]]

Current revision

Crystal structure of E. coli regulator of ribonuclease activity A (RraA) bound to fragment of DEAD-box protein RhlB

PDB ID 2yjv

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