3vyl
From Proteopedia
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| - | {{STRUCTURE_3vyl| PDB=3vyl | SCENE= }} | ||
| - | ===Structure of L-ribulose 3-epimerase=== | ||
| - | == | + | ==Structure of L-ribulose 3-epimerase== |
| - | [[3vyl]] is a 8 chain structure with sequence from [ | + | <StructureSection load='3vyl' size='340' side='right'caption='[[3vyl]], [[Resolution|resolution]] 2.70Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3vyl]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VYL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VYL FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vyl OCA], [https://pdbe.org/3vyl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vyl RCSB], [https://www.ebi.ac.uk/pdbsum/3vyl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vyl ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/N0DP12_RHILI N0DP12_RHILI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | L-Ribulose 3-epimerase (L-RE) from Mesorhizobium loti has been identified as the first ketose 3-epimerase that shows the highest observed activity towards ketopentoses. In the present study, the crystal structure of the enzyme was determined to 2.7 A resolution. The asymmetric unit contained two homotetramers with the monomer folded into an (alpha/beta)8-barrel carrying four additional short alpha-helices. The overall structure of M. loti L-RE showed significant similarity to the structures of ketose 3-epimerases from Pseudomonas cichorii, Agrobacterium tumefaciens and Clostridium cellulolyticum, which use ketohexoses as preferred substrates. However, the size of the C-terminal helix (alpha8) was much larger in M. loti L-RE than the corresponding helices in the other enzymes. In M. loti L-RE the alpha8 helix and the following C-terminal tail possessed a unique subunit-subunit interface which promoted the formation of additional intermolecular interactions and strengthened the enzyme stability. Structural comparisons revealed that the relatively small hydrophobic pocket of the enzyme around the substrate was likely to be the main factor responsible for the marked specificity for ketopentoses shown by M. loti L-RE. | ||
| + | |||
| + | Structural insight into L-ribulose 3-epimerase from Mesorhizobium loti.,Uechi K, Sakuraba H, Yoshihara A, Morimoto K, Takata G Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2330-9. doi:, 10.1107/S0907444913021665. Epub 2013 Nov 19. PMID:24311575<ref>PMID:24311575</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3vyl" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Mesorhizobium loti]] | [[Category: Mesorhizobium loti]] | ||
| - | [[Category: Sakuraba | + | [[Category: Sakuraba H]] |
| - | [[Category: Takata | + | [[Category: Takata G]] |
| - | [[Category: Uechi | + | [[Category: Uechi K]] |
| - | + | ||
| - | + | ||
Current revision
Structure of L-ribulose 3-epimerase
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