4fwt

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{{STRUCTURE_4fwt| PDB=4fwt | SCENE= }}
 
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===Complex structure of viral RNA polymerase form III===
 
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{{ABSTRACT_PUBMED_22884418}}
 
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==Function==
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==Complex structure of viral RNA polymerase form III==
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[[http://www.uniprot.org/uniprot/EFTS_ECO57 EFTS_ECO57]] Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome (By similarity).
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<StructureSection load='4fwt' size='340' side='right'caption='[[4fwt]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4fwt]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7] and [https://en.wikipedia.org/wiki/Escherichia_virus_Qbeta Escherichia virus Qbeta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FWT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fwt OCA], [https://pdbe.org/4fwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fwt RCSB], [https://www.ebi.ac.uk/pdbsum/4fwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fwt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EFTU_ECO57 EFTU_ECO57] [https://www.uniprot.org/uniprot/RDRP_BPQBE RDRP_BPQBE] This enzyme is part of the viral RNA-dependent RNA polymerase complex.[https://www.uniprot.org/uniprot/EFTS_ECO57 EFTS_ECO57] Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The genomic RNA of Qbeta virus is replicated by Qbeta replicase, a template-dependent RNA polymerase complex. Qbeta replicase has an intrinsic template-independent RNA 3'-adenylation activity, which is required for efficient viral RNA amplification in the host cells. However, the mechanism of the template-independent 3'-adenylation of RNAs by Qbeta replicase has remained elusive. We determined the structure of a complex that includes Qbeta replicase, a template RNA, a growing RNA complementary to the template RNA, and ATP. The structure represents the terminal stage of RNA polymerization and reveals that the shape and size of the nucleotide-binding pocket becomes available for ATP accommodation after the 3'-penultimate template-dependent C-addition. The stacking interaction between the ATP and the neighboring Watson-Crick base pair, between the 5'-G in the template and the 3'-C in the growing RNA, contributes to the nucleotide specificity. Thus, the template for the template-independent 3'-adenylation by Qbeta replicase is the RNA and protein ribonucleoprotein complex.
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==About this Structure==
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Mechanism for template-independent terminal adenylation activity of Qbeta replicase.,Takeshita D, Yamashita S, Tomita K Structure. 2012 Oct 10;20(10):1661-9. doi: 10.1016/j.str.2012.07.004. Epub 2012, Aug 9. PMID:22884418<ref>PMID:22884418</ref>
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[[4fwt]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FWT OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:022884418</ref><references group="xtra"/><references/>
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</div>
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[[Category: Synthetic construct]]
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<div class="pdbe-citations 4fwt" style="background-color:#fffaf0;"></div>
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[[Category: Takeshita, D.]]
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[[Category: Tomita, K.]]
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==See Also==
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[[Category: Rna polymerase]]
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*[[Elongation factor 3D structures|Elongation factor 3D structures]]
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[[Category: Transferase-rna complex]]
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== References ==
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[[Category: Translation]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli O157:H7]]
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[[Category: Escherichia virus Qbeta]]
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[[Category: Large Structures]]
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[[Category: Takeshita D]]
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[[Category: Tomita K]]

Current revision

Complex structure of viral RNA polymerase form III

PDB ID 4fwt

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