4mgm
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the in vitro transcribed G. kaustophilus tRNA-Gly== | |
| + | <StructureSection load='4mgm' size='340' side='right'caption='[[4mgm]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4mgm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MGM FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mgm OCA], [https://pdbe.org/4mgm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mgm RCSB], [https://www.ebi.ac.uk/pdbsum/4mgm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mgm ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | T box riboswitches are cis-acting RNA elements that bind to tRNA and sense its aminoacylation state to influence gene expression. Here, we present the 3.2 A resolution X-ray crystal structures of the T box Stem I-tRNA complex and tRNA, in isolation. T box Stem I forms an arched conformation with extensive intermolecular contacts to two key points of tRNA, the anticodon and D/T-loops. Free and complexed tRNA exist in significantly different conformations, with the contacts stabilizing flexible D/T-loops and a rearrangement of the D-loop. Using a designed T box RNA/tRNA system, we demonstrate that the T box riboswitch monitors the length and orientation of two essential contacts. Length or orientation mismatches engineered into the T box riboswitch and tRNA disrupt the complex, whereas simultaneous insertion of full helical turns realigns the interfaces and restores interaction between artificially elongated T box riboswitch and tRNA molecules. | ||
| - | + | Structural Determinants for Geometry and Information Decoding of tRNA by T Box Leader RNA.,Grigg JC, Ke A Structure. 2013 Oct 1. pii: S0969-2126(13)00346-8. doi:, 10.1016/j.str.2013.09.001. PMID:24095061<ref>PMID:24095061</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4mgm" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Geobacillus kaustophilus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Grigg JC]] | ||
| + | [[Category: Ke A]] | ||
Current revision
Crystal structure of the in vitro transcribed G. kaustophilus tRNA-Gly
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