2ow4

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[[Image:2ow4.jpg|left|200px]]<br /><applet load="2ow4" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ow4, resolution 1.60&Aring;" />
 
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'''Crystal structure of a lectin from Canavalia maritima seeds (ConM) in complex with man1-2man-OMe'''<br />
 
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==Overview==
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==Crystal structure of a lectin from Canavalia maritima seeds (ConM) in complex with man1-2man-OMe==
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Plant lectins, especially those purified from species of the Leguminosae family, represent the best studied group of carbohydrate-binding proteins. The legume lectins from Diocleinae subtribe are highly similar proteins that present significant differences in the potency/efficacy of their biological activities. The structural studies of the interactions between lectins and sugars may clarify the origin of the distinct biological activities observed in this high similar class of proteins. In this way, this work presents a crystallographic study of the ConM and CGL (agglutinins from Canavalia maritima and Canavalia gladiata, respectively) in the following complexes: ConM/CGL:Man(alpha1-2)Man(alpha1-O)Me, ConM/CGL:Man(alpha1-3)Man(alpha1-O)Me and ConM/CGL:Man(alpha1-4)Man(alpha1-O)Me, which crystallized in different conditions and space group from the native proteins. The structures were solved by molecular replacement, presenting satisfactory values for R(factor) and R(free). Comparisons between ConM, CGL and ConA (Canavalia ensiformis lectin) binding mode with the dimannosides in subject, presented different interactions patterns, which may account for a structural explanation of the distincts biological properties observed in the lectins of Diocleinae subtribe.
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<StructureSection load='2ow4' size='340' side='right'caption='[[2ow4]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ow4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_rosea Canavalia rosea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OW4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OW4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ow4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ow4 OCA], [https://pdbe.org/2ow4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ow4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ow4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ow4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CONA_CANRO CONA_CANRO] Glucose/D-mannose specific lectin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ow/2ow4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ow4 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2OW4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Canavalia_maritima Canavalia maritima] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OW4 OCA].
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*[[Concanavalin 3D structures|Concanavalin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural analysis of Canavalia maritima and Canavalia gladiata lectins complexed with different dimannosides: new insights into the understanding of the structure-biological activity relationship in legume lectins., Bezerra GA, Oliveira TM, Moreno FB, de Souza EP, da Rocha BA, Benevides RG, Delatorre P, de Azevedo WF Jr, Cavada BS, J Struct Biol. 2007 Nov;160(2):168-76. Epub 2007 Aug 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17881248 17881248]
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[[Category: Canavalia rosea]]
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[[Category: Canavalia maritima]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Benevides RG]]
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[[Category: Benevides, R G.]]
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[[Category: Bezerra GA]]
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[[Category: Bezerra, G A.]]
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[[Category: Cavada BS]]
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[[Category: Cavada, B S.]]
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[[Category: Delatorre P]]
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[[Category: Delatorre, P.]]
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[[Category: Moreno FBMB]]
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[[Category: Jr., W F.de Azevedo.]]
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[[Category: Da Rocha BAM]]
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[[Category: Moreno, F B.M B.]]
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[[Category: De Azevedo Jr WF]]
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[[Category: Oliveira, T M.de.]]
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[[Category: De Oliveira TM]]
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[[Category: Rocha, B A.M da.]]
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[[Category: De Souza EM]]
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[[Category: Souza, E M.de.]]
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[[Category: CA]]
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[[Category: MN]]
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[[Category: canavalia maritima]]
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[[Category: lectin]]
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[[Category: man1-2man-ome]]
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[[Category: sugar binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:23:12 2008''
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Current revision

Crystal structure of a lectin from Canavalia maritima seeds (ConM) in complex with man1-2man-OMe

PDB ID 2ow4

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