4bmc

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{{STRUCTURE_4bmc| PDB=4bmc | SCENE= }}
 
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===Crystal structure of s.pombe Rad4 BRCT1,2===
 
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{{ABSTRACT_PUBMED_24074952}}
 
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==Function==
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==Crystal structure of s.pombe Rad4 BRCT1,2==
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[[http://www.uniprot.org/uniprot/RAD4_SCHPO RAD4_SCHPO]] Essential component for DNA replication and also the checkpoint control system which couples S and M phases. May directly or indirectly interact with chromatin proteins to form the complex required for the initiation and/or progression of DNA synthesis.
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<StructureSection load='4bmc' size='340' side='right'caption='[[4bmc]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4bmc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BMC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BMC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.982&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bmc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bmc OCA], [https://pdbe.org/4bmc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bmc RCSB], [https://www.ebi.ac.uk/pdbsum/4bmc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bmc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RAD4_SCHPO RAD4_SCHPO] Essential component for DNA replication and also the checkpoint control system which couples S and M phases. May directly or indirectly interact with chromatin proteins to form the complex required for the initiation and/or progression of DNA synthesis.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The BRCT-domain protein Rad4(TopBP1) facilitates activation of the DNA damage checkpoint in Schizosaccharomyces pombe by physically coupling the Rad9-Rad1-Hus1 clamp, the Rad3(ATR) -Rad26(ATRIP) kinase complex, and the Crb2(53BP1) mediator. We have now determined crystal structures of the BRCT repeats of Rad4(TopBP1), revealing a distinctive domain architecture, and characterized their phosphorylation-dependent interactions with Rad9 and Crb2(53BP1). We identify a cluster of phosphorylation sites in the N-terminal region of Crb2(53BP1) that mediate interaction with Rad4(TopBP1) and reveal a hierarchical phosphorylation mechanism in which phosphorylation of Crb2(53BP1) residues Thr215 and Thr235 promotes phosphorylation of the noncanonical Thr187 site by scaffolding cyclin-dependent kinase (CDK) recruitment. Finally, we show that the simultaneous interaction of a single Rad4(TopBP1) molecule with both Thr187 phosphorylation sites in a Crb2(53BP1) dimer is essential for establishing the DNA damage checkpoint.
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==About this Structure==
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Phosphorylation-Dependent Assembly and Coordination of the DNA Damage Checkpoint Apparatus by Rad4(TopBP1.).,Qu M, Rappas M, Wardlaw CP, Garcia V, Ren JY, Day M, Carr AM, Oliver AW, Du LL, Pearl LH Mol Cell. 2013 Sep 26;51(6):723-36. doi: 10.1016/j.molcel.2013.08.030. PMID:24074952<ref>PMID:24074952</ref>
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[[4bmc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BMC OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024074952</ref><references group="xtra"/><references/>
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</div>
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<div class="pdbe-citations 4bmc" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[DNA repair protein Rad4|DNA repair protein Rad4]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
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[[Category: Carr, A M.]]
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[[Category: Carr AM]]
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[[Category: Du, L L.]]
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[[Category: Du LL]]
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[[Category: Garcia, V.]]
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[[Category: Garcia V]]
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[[Category: Meng, Q.]]
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[[Category: Meng Q]]
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[[Category: Oliver, A W.]]
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[[Category: Oliver AW]]
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[[Category: Pearl, L H.]]
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[[Category: Pearl LH]]
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[[Category: Rappas, M.]]
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[[Category: Rappas M]]
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[[Category: Wardlaw, C P.]]
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[[Category: Wardlaw CP]]
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[[Category: Dna damage checkpoint]]
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[[Category: Replication]]
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[[Category: Topbp1]]
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Current revision

Crystal structure of s.pombe Rad4 BRCT1,2

PDB ID 4bmc

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