4k6n

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{{STRUCTURE_4k6n| PDB=4k6n | SCENE= }}
 
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===Crystal structure of yeast 4-amino-4-deoxychorismate lyase===
 
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{{ABSTRACT_PUBMED_23818518}}
 
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==Function==
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==Crystal structure of yeast 4-amino-4-deoxychorismate lyase==
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[[http://www.uniprot.org/uniprot/PABC_YEAST PABC_YEAST]] Converts 4-amino-4-deoxychorismate into 4-aminobenzoate (PABA) and pyruvate (By similarity).
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<StructureSection load='4k6n' size='340' side='right'caption='[[4k6n]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4k6n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K6N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K6N FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k6n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k6n OCA], [https://pdbe.org/4k6n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k6n RCSB], [https://www.ebi.ac.uk/pdbsum/4k6n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k6n ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PABC_YEAST PABC_YEAST] Converts 4-amino-4-deoxychorismate into 4-aminobenzoate (PABA) and pyruvate (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Saccharomyces cerevisiae Abz2 is a pyridoxal 5'-phosphate (PLP)-dependent lyase that converts 4-amino-4-deoxychorismate (ADC) to para-aminobenzoate and pyruvate. To investigate the catalytic mechanism, we determined the 1.9 A resolution crystal structure of Abz2 complexed with PLP, representing the first eukaryotic ADC lyase structure. Unlike Escherichia coli ADC lyase, whose dimerization is critical to the formation of the active site, the overall structure of Abz2 displays as a monomer of two domains. At the interdomain cleft, a molecule of cofactor PLP forms a Schiff base with residue Lys-251. Computational simulations defined a basic clamp to orientate the substrate ADC in a proper pose, which was validated by site-directed mutageneses combined with enzymatic activity assays. Altogether, we propose a putative catalytic mechanism of a unique class of monomeric ADC lyases led by yeast Abz2.
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==About this Structure==
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Structure and catalytic mechanism of yeast 4-amino-4-deoxychorismate lyase.,Dai YN, Chi CB, Zhou K, Cheng W, Jiang YL, Ren YM, Ruan K, Chen Y, Zhou CZ J Biol Chem. 2013 Aug 9;288(32):22985-92. doi: 10.1074/jbc.M113.480335. Epub 2013, Jul 1. PMID:23818518<ref>PMID:23818518</ref>
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[[4k6n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K6N OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023818518</ref><references group="xtra"/><references/>
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</div>
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[[Category: Aminodeoxychorismate lyase]]
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<div class="pdbe-citations 4k6n" style="background-color:#fffaf0;"></div>
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[[Category: Saccharomyces cerevisiae s288c]]
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== References ==
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[[Category: Chen, Y.]]
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<references/>
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[[Category: Cheng, W.]]
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__TOC__
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[[Category: Chi, C B.]]
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</StructureSection>
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[[Category: Dai, Y N.]]
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[[Category: Large Structures]]
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[[Category: Jiang, Y L.]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Ren, Y M.]]
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[[Category: Chen Y]]
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[[Category: Zhou, C Z.]]
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[[Category: Cheng W]]
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[[Category: Zhou, K.]]
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[[Category: Chi C-B]]
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[[Category: 4-amino-4-deoxychorismate]]
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[[Category: Dai Y-N]]
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[[Category: Lyase]]
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[[Category: Jiang Y-L]]
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[[Category: Plp]]
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[[Category: Ren Y-M]]
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[[Category: Plp-dependent fold type iv enzyme]]
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[[Category: Zhou C-Z]]
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[[Category: Pyruvate]]
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[[Category: Zhou K]]

Current revision

Crystal structure of yeast 4-amino-4-deoxychorismate lyase

PDB ID 4k6n

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