2rsg

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{{STRUCTURE_2rsg| PDB=2rsg | SCENE= }}
 
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===Solution structure of the CERT PH domain===
 
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{{ABSTRACT_PUBMED_22869376}}
 
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==Function==
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==Solution structure of the CERT PH domain==
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[[http://www.uniprot.org/uniprot/C43BP_HUMAN C43BP_HUMAN]] Shelters ceramides and diacylglycerol lipids inside its START domain and mediates the intracellular trafficking of ceramides and diacylglycerol lipids in a non-vesicular manner.<ref>PMID:14685229</ref> <ref>PMID:17591919</ref> <ref>PMID:18184806</ref> <ref>PMID:20036255</ref>
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<StructureSection load='2rsg' size='340' side='right'caption='[[2rsg]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2rsg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RSG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RSG FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rsg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rsg OCA], [https://pdbe.org/2rsg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rsg RCSB], [https://www.ebi.ac.uk/pdbsum/2rsg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rsg ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/CERT_HUMAN CERT_HUMAN] The disease is caused by variants affecting the gene represented in this entry.
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== Function ==
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[https://www.uniprot.org/uniprot/CERT_HUMAN CERT_HUMAN] Shelters ceramides and diacylglycerol lipids inside its START domain and mediates the intracellular trafficking of ceramides and diacylglycerol lipids in a non-vesicular manner.<ref>PMID:14685229</ref> <ref>PMID:17591919</ref> <ref>PMID:18184806</ref> <ref>PMID:20036255</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ceramide transport from the endoplasmic reticulum to the Golgi apparatus is crucial in sphingolipid biosynthesis, and the process relies on the ceramide trafficking protein (CERT), which contains pleckstrin homology (PH) and StAR-related lipid transfer domains. The CERT PH domain specifically recognizes phosphatidylinositol 4-monophosphate (PtdIns(4)P), a characteristic phosphoinositide in the Golgi membrane, and is indispensable for the endoplasmic reticulum-to-Golgi transport of ceramide by CERT. In this study, we determined the three-dimensional structure of the CERT PH domain by using solution NMR techniques. The structure revealed the presence of a characteristic basic groove near the canonical PtdIns(4)P recognition site. An extensive interaction study using NMR and other biophysical techniques revealed that the basic groove coordinates the CERT PH domain for efficient PtdIns(4)P recognition and localization in the Golgi apparatus. The notion was also supported by Golgi mislocalization of the CERT mutants in living cells. The distinctive binding modes reflect the functions of PH domains, as the basic groove is conserved only in the PH domains involved with the PtdIns(4)P-dependent lipid transport activity but not in those with the signal transduction activity.
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==About this Structure==
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Structural basis for the Golgi association by the pleckstrin homology domain of the ceramide trafficking protein (CERT).,Sugiki T, Takeuchi K, Yamaji T, Takano T, Tokunaga Y, Kumagai K, Hanada K, Takahashi H, Shimada I J Biol Chem. 2012 Sep 28;287(40):33706-18. Epub 2012 Aug 6. PMID:22869376<ref>PMID:22869376</ref>
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[[2rsg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RSG OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:022869376</ref><references group="xtra"/><references/>
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</div>
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<div class="pdbe-citations 2rsg" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Hanada, K.]]
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[[Category: Large Structures]]
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[[Category: Kawano, M.]]
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[[Category: Hanada K]]
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[[Category: Kumagai, K.]]
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[[Category: Kawano M]]
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[[Category: Nishijima, M.]]
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[[Category: Kumagai K]]
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[[Category: Shimada, I.]]
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[[Category: Nishijima M]]
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[[Category: Sugiki, T.]]
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[[Category: Shimada I]]
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[[Category: Takahashi, H.]]
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[[Category: Sugiki T]]
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[[Category: Takeuchi, K.]]
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[[Category: Takahashi H]]
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[[Category: Tokunaga, Y.]]
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[[Category: Takeuchi K]]
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[[Category: Lipid transport]]
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[[Category: Tokunaga Y]]
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[[Category: Pleckstrin homology]]
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Current revision

Solution structure of the CERT PH domain

PDB ID 2rsg

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