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2p0b

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[[Image:2p0b.jpg|left|200px]]<br /><applet load="2p0b" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2p0b, resolution 1.740&Aring;" />
 
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'''Crystal structure of chemically-reduced E.coli nrfB'''<br />
 
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==Overview==
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==Crystal structure of chemically-reduced E.coli nrfB==
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NrfB is a small pentahaem electron-transfer protein widely involved in the respiratory reduction of nitrite or nitric oxide to ammonia, processes that provide energy for anaerobic metabolism in many enteric bacteria and also serve to detoxify these reactive nitrogen species. The X-ray crystal structure of Escherichia coli NrfB is presented at 1.74 A (1 A=0.1 nm) resolution. The architecture of the protein is that of a 40 A 'nanowire' in which the five haems are positioned within 6 A of each other along a polypeptide scaffold. During nitrite reduction, the physiological role of NrfB is to mediate electron transfer to another pentahaem protein, NrfA, the enzyme that catalyses periplasmic nitrite or nitric oxide reduction. Protein-protein interaction studies suggest NrfA and NrfB can form a 20-haem NrfA2-NrfB2 heterotetrameric complex.
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<StructureSection load='2p0b' size='340' side='right'caption='[[2p0b]], [[Resolution|resolution]] 1.74&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2p0b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P0B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P0B FirstGlance]. <br>
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2P0B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=HEC:'>HEC</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P0B OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.74&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p0b OCA], [https://pdbe.org/2p0b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p0b RCSB], [https://www.ebi.ac.uk/pdbsum/2p0b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p0b ProSAT]</span></td></tr>
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The crystal structure of the pentahaem c-type cytochrome NrfB and characterization of its solution-state interaction with the pentahaem nitrite reductase NrfA., Clarke TA, Cole JA, Richardson DJ, Hemmings AM, Biochem J. 2007 Aug 15;406(1):19-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17521287 17521287]
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</table>
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[[Category: Escherichia coli]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/NRFB_ECOLI NRFB_ECOLI] Plays a role in nitrite reduction.
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[[Category: Clarke, T A.]]
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== Evolutionary Conservation ==
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[[Category: Hemmings, A M.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Richardson, D J.]]
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Check<jmol>
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[[Category: HEC]]
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<jmolCheckbox>
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[[Category: electron transport]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p0/2p0b_consurf.spt"</scriptWhenChecked>
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[[Category: pentaheme c-type cytochrome]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:24:28 2008''
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p0b ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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[[Category: Clarke TA]]
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[[Category: Hemmings AM]]
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[[Category: Richardson DJ]]

Current revision

Crystal structure of chemically-reduced E.coli nrfB

PDB ID 2p0b

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