4it3

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{{STRUCTURE_4it3| PDB=4it3 | SCENE= }}
 
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===Crystal Structure of Iml3 from S. cerevisiae===
 
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{{ABSTRACT_PUBMED_24075991}}
 
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==Function==
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==Crystal Structure of Iml3 from S. cerevisiae==
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[[http://www.uniprot.org/uniprot/IML3_YEAST IML3_YEAST]] Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.<ref>PMID:11361335</ref> <ref>PMID:15241623</ref>
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<StructureSection load='4it3' size='340' side='right'caption='[[4it3]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4it3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IT3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IT3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.495&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4it3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4it3 OCA], [https://pdbe.org/4it3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4it3 RCSB], [https://www.ebi.ac.uk/pdbsum/4it3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4it3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CENPL_YEAST CENPL_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.<ref>PMID:11361335</ref> <ref>PMID:15241623</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Accurate segregation of genetic material in eukaryotes relies on the kinetochore, a multiprotein complex that connects centromeric DNA with microtubules. In yeast and humans, two proteins-Mif2/CENP-C and Chl4/CNEP-N-interact with specialized centromeric nucleosomes and establish distinct but cross-connecting axes of chromatin-microtubule linkage. Proteins recruited by Chl4/CENP-N include a subset that regulates chromosome transmission fidelity. We show that Chl4 and a conserved member of this subset, Iml3, both from Saccharomyces cerevisiae, form a stable protein complex that interacts with Mif2 and Sgo1. We have determined the structures of an Iml3 homodimer and an Iml3-Chl4 heterodimer, which suggest a mechanism for regulating the assembly of this functional axis of the kinetochore. We propose that at the core centromere, the Chl4-Iml3 complex participates in recruiting factors, such as Sgo1, that influence sister chromatid cohesion and encourage sister kinetochore biorientation.
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==About this Structure==
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An Iml3-Chl4 Heterodimer Links the Core Centromere to Factors Required for Accurate Chromosome Segregation.,Hinshaw SM, Harrison SC Cell Rep. 2013 Sep 25. pii: S2211-1247(13)00477-4. doi:, 10.1016/j.celrep.2013.08.036. PMID:24075991<ref>PMID:24075991</ref>
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[[4it3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IT3 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024075991</ref><references group="xtra"/><references/>
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</div>
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<div class="pdbe-citations 4it3" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Harrison, S C.]]
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[[Category: Harrison SC]]
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[[Category: Hinshaw, S M.]]
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[[Category: Hinshaw SM]]
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[[Category: Beta sheet]]
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[[Category: Cell cycle]]
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[[Category: Chl4]]
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[[Category: Kinetochore]]
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[[Category: Nucleus]]
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Current revision

Crystal Structure of Iml3 from S. cerevisiae

PDB ID 4it3

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