4j0l

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{{STRUCTURE_4j0l| PDB=4j0l | SCENE= }}
 
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===Crystal Structure of a Family GH19 Chitinase (W72A/E67Q mutant) from rye seeds in complex with two (GlcNAc)4 molecules===
 
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{{ABSTRACT_PUBMED_23871710}}
 
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==Function==
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==Crystal Structure of a Family GH19 Chitinase (W72A/E67Q mutant) from rye seeds in complex with two (GlcNAc)4 molecules==
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[[http://www.uniprot.org/uniprot/CHIC_SECCE CHIC_SECCE]] Defense against chitin containing fungal pathogens. Binds the hyphal tips of fungi and degrades nascent chitin.<ref>PMID:11999399</ref> <ref>PMID:11826968</ref> <ref>PMID:12092848</ref>
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<StructureSection load='4j0l' size='340' side='right'caption='[[4j0l]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4j0l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Secale_cereale Secale cereale]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J0L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J0L FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j0l OCA], [https://pdbe.org/4j0l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j0l RCSB], [https://www.ebi.ac.uk/pdbsum/4j0l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j0l ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CHIC_SECCE CHIC_SECCE] Defense against chitin containing fungal pathogens. Binds the hyphal tips of fungi and degrades nascent chitin.<ref>PMID:11999399</ref> <ref>PMID:11826968</ref> <ref>PMID:12092848</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Crystallographic analysis of a mutated form of "loopful" GH19 chitinase from rye seeds a double mutant RSC-c, in which Glu67 and Trp72 are mutated to glutamine and alanine, respectively, (RSC-c-E67Q/W72A) in complex with chitin tetrasaccharide (GlcNAc)(4) revealed that the entire substrate-binding cleft was completely occupied with the sugar residues of two (GlcNAc)(4) molecules. One (GlcNAc)(4) molecule bound to subsites -4 to -1, while the other bound to subsites +1 to +4. Comparisons of the main chain conformation between liganded RSC-c-E67Q/W72A and unliganded wild type RSC-c suggested domain motion essential for catalysis. This is the first report on the complete subsite mapping of GH19 chitinase.
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==About this Structure==
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Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure.,Ohnuma T, Umemoto N, Kondo K, Numata T, Fukamizo T FEBS Lett. 2013 Aug 19;587(16):2691-7. doi: 10.1016/j.febslet.2013.07.008. Epub, 2013 Jul 18. PMID:23871710<ref>PMID:23871710</ref>
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[[4j0l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Secale_cereale Secale cereale]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J0L OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023871710</ref><references group="xtra"/><references/>
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</div>
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[[Category: Chitinase]]
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<div class="pdbe-citations 4j0l" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Chitinase 3D structures|Chitinase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Secale cereale]]
[[Category: Secale cereale]]
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[[Category: Fukamizo, T.]]
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[[Category: Fukamizo T]]
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[[Category: Numata, T.]]
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[[Category: Numata T]]
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[[Category: Ohnuma, T.]]
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[[Category: Ohnuma T]]
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[[Category: Umemoto, N.]]
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[[Category: Umemoto N]]
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[[Category: Carbohydrate]]
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[[Category: Chitinase]]
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[[Category: Hydrolase]]
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Current revision

Crystal Structure of a Family GH19 Chitinase (W72A/E67Q mutant) from rye seeds in complex with two (GlcNAc)4 molecules

PDB ID 4j0l

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