4bwj
From Proteopedia
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| - | {{STRUCTURE_4bwj| PDB=4bwj | SCENE= }} | ||
| - | ===KlenTaq mutant in complex with DNA and ddCTP=== | ||
| - | {{ABSTRACT_PUBMED_24106012}} | ||
| - | == | + | ==KlenTaq mutant in complex with DNA and ddCTP== |
| - | [[4bwj]] is a 3 chain structure with sequence from [ | + | <StructureSection load='4bwj' size='340' side='right'caption='[[4bwj]], [[Resolution|resolution]] 1.55Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4bwj]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BWJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BWJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DCT:2,3-DIDEOXYCYTIDINE+5-TRIPHOSPHATE'>DCT</scene>, <scene name='pdbligand=DOC:2,3-DIDEOXYCYTIDINE-5-MONOPHOSPHATE'>DOC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bwj OCA], [https://pdbe.org/4bwj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bwj RCSB], [https://www.ebi.ac.uk/pdbsum/4bwj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bwj ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/DPO1_THEAQ DPO1_THEAQ] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Unfaithful: The structure of a DNA-dependent DNA polymerase, whose parental ancestor has no significant reverse transcriptase activity, was obtained for the first time incorporating a nucleotide against an RNA template and then structurally characterized. The DNA polymerase variant has significant reverse transcriptase activity that allows its application in PCR-based molecular diagnostics. | ||
| - | + | Structure and Function of an RNA-Reading Thermostable DNA Polymerase.,Blatter N, Bergen K, Nolte O, Welte W, Diederichs K, Mayer J, Wieland M, Marx A Angew Chem Int Ed Engl. 2013 Sep 17. doi: 10.1002/anie.201306655. PMID:24106012<ref>PMID:24106012</ref> | |
| - | <ref | + | |
| - | [[Category: | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| + | </div> | ||
| + | <div class="pdbe-citations 4bwj" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[DNA polymerase 3D structures|DNA polymerase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
[[Category: Thermus aquaticus]] | [[Category: Thermus aquaticus]] | ||
| - | [[Category: Bergen | + | [[Category: Bergen K]] |
| - | [[Category: Blatter | + | [[Category: Blatter N]] |
| - | [[Category: Diederichs | + | [[Category: Diederichs K]] |
| - | [[Category: Marx | + | [[Category: Marx A]] |
| - | [[Category: Welte | + | [[Category: Welte W]] |
| - | + | ||
| - | + | ||
Current revision
KlenTaq mutant in complex with DNA and ddCTP
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