3st0

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{{STRUCTURE_3st0| PDB=3st0 | SCENE= }}
 
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===Engineered medium-affinity halide-binding protein derived from YFP: halide-free===
 
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{{ABSTRACT_PUBMED_24107961}}
 
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==Function==
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==Engineered medium-affinity halide-binding protein derived from YFP: halide-free==
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[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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<StructureSection load='3st0' size='340' side='right'caption='[[3st0]], [[Resolution|resolution]] 1.19&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3st0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ST0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ST0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.19&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3st0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3st0 OCA], [https://pdbe.org/3st0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3st0 RCSB], [https://www.ebi.ac.uk/pdbsum/3st0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3st0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We describe an engineered fluorescent optogenetic sensor, SuperClomeleon, that robustly detects inhibitory synaptic activity in single, cultured mouse neurons by reporting intracellular chloride changes produced by exogenous GABA or inhibitory synaptic activity. Using a cell-free protein engineering automation methodology that bypasses gene cloning, we iteratively constructed, produced, and assayed hundreds of mutations in binding-site residues to identify improvements in Clomeleon, a first-generation, suboptimal sensor. Structural analysis revealed that these improvements involve halide contacts and distant side chain rearrangements. The development of optogenetic sensors that respond to neural activity enables cellular tracking of neural activity using optical, rather than electrophysiological, signals. Construction of such sensors using in vitro protein engineering establishes a powerful approach for developing new probes for brain imaging.
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==About this Structure==
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Visualization of synaptic inhibition with an optogenetic sensor developed by cell-free protein engineering automation.,Grimley JS, Li L, Wang W, Wen L, Beese LS, Hellinga HW, Augustine GJ J Neurosci. 2013 Oct 9;33(41):16297-309. doi: 10.1523/JNEUROSCI.4616-11.2013. PMID:24107961<ref>PMID:24107961</ref>
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[[3st0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ST0 OCA].
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==See Also==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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*[[Green Fluorescent Protein|Green Fluorescent Protein]]
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</div>
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<div class="pdbe-citations 3st0" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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<ref group="xtra">PMID:024107961</ref><references group="xtra"/><references/>
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
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[[Category: Beese, L S.]]
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[[Category: Large Structures]]
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[[Category: Grimley, J S.]]
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[[Category: Beese LS]]
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[[Category: Hellinga, H W.]]
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[[Category: Grimley JS]]
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[[Category: Wang, W.]]
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[[Category: Hellinga HW]]
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[[Category: Beta barrel]]
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[[Category: Wang W]]
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[[Category: Halide binding protein]]
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[[Category: Imaging reagent]]
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[[Category: Luminescent protein]]
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[[Category: Yellow fluorescent protein]]
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Current revision

Engineered medium-affinity halide-binding protein derived from YFP: halide-free

PDB ID 3st0

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