|
m |
| (11 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:1w8r.gif|left|200px]]<br /> | + | #REDIRECT [[2yce]] This PDB entry is obsolete and replaced by 2yce |
| - | <applet load="1w8r" size="450" color="white" frame="true" align="right" spinBox="true"
| + | |
| - | caption="1w8r, resolution 1.93Å" />
| + | |
| - | '''THE MECHANISM OF THE SCHIFF BASE FORMING FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE: STRUCTURAL ANALYSIS OF REACTION INTERMEDIATES'''<br />
| + | |
| - | | + | |
| - | ==Overview==
| + | |
| - | The glycolytic enzyme fructose-1,6-bisphosphate aldolase (FBPA) catalyzes, the reversible cleavage of fructose 1,6-bisphosphate to glyceraldehyde, 3-phosphate and dihydroxyacetone phosphate. Catalysis of Schiff base, forming class I FBPA relies on a number of intermediates covalently bound, to the catalytic lysine. Using active site mutants of FBPA I from, Thermoproteus tenax, we have solved the crystal structures of the enzyme, covalently bound to the carbinolamine of the substrate fructose, 1,6-bisphosphate and noncovalently bound to the cyclic form of the, substrate. The structures, determined at a resolution of 1.9 A and refined, to crystallographic R factors of 0.148 and 0.149, respectively, represent, the first view of any FBPA I in these two stages of the reaction pathway, and ... [[http://ispc.weizmann.ac.il/pmbin/getpm?15766250 (full description)]]
| + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1W8R is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermoproteus_tenax Thermoproteus tenax]] with F2P as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W8R OCA]].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | Mechanism of the Schiff base forming fructose-1,6-bisphosphate aldolase: structural analysis of reaction intermediates., Lorentzen E, Siebers B, Hensel R, Pohl E, Biochemistry. 2005 Mar 22;44(11):4222-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15766250 15766250]
| + | |
| - | [[Category: Fructose-bisphosphate aldolase]]
| + | |
| - | [[Category: Single protein]]
| + | |
| - | [[Category: Thermoproteus tenax]]
| + | |
| - | [[Category: Hensel, R.]]
| + | |
| - | [[Category: Lorentzen, E.]]
| + | |
| - | [[Category: Pohl, E.]]
| + | |
| - | [[Category: Siebers, B.]]
| + | |
| - | [[Category: F2P]]
| + | |
| - | [[Category: 6-bisphosphate]]
| + | |
| - | [[Category: aldolase]]
| + | |
| - | [[Category: archaeal]]
| + | |
| - | [[Category: catalytic mechanism]]
| + | |
| - | [[Category: fructose 1]]
| + | |
| - | [[Category: glycolytic]]
| + | |
| - | [[Category: lyase]]
| + | |
| - | [[Category: reaction intermediate]]
| + | |
| - | [[Category: tim barrel]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:28:40 2007''
| + | |
