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4mn4
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| - | {{STRUCTURE_4mn4| PDB=4mn4 | SCENE= }} | ||
| - | ===Structural Basis for the MukB-topoisomerase IV Interaction=== | ||
| - | {{ABSTRACT_PUBMED_24097060}} | ||
| - | == | + | ==Structural Basis for the MukB-topoisomerase IV Interaction== |
| - | [[http://www.uniprot.org/uniprot/PARC_ECOLI PARC_ECOLI | + | <StructureSection load='4mn4' size='340' side='right'caption='[[4mn4]], [[Resolution|resolution]] 2.30Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4mn4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._MG1655 Escherichia coli str. K-12 substr. MG1655]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MN4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MN4 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mn4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mn4 OCA], [https://pdbe.org/4mn4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mn4 RCSB], [https://www.ebi.ac.uk/pdbsum/4mn4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mn4 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PARC_ECOLI PARC_ECOLI] Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule. MukB stimulates the relaxation activity of topoisomerase IV and also has a modest effect on decatenation.<ref>PMID:20921377</ref> <ref>PMID:12269820</ref> <ref>PMID:21300644</ref> <ref>PMID:16023670</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Chromosome partitioning in Escherichia coli is assisted by two interacting proteins, topoisomerase (topo) IV and MukB. MukB stimulates the relaxation of negative supercoils by topo IV; to understand the mechanism of their action and to define this functional interplay, we determined the crystal structure of a minimal MukB-topo IV complex to 2.3 A resolution. The structure shows that the so-called 'hinge' region of MukB forms a heterotetrameric assembly with a C-terminal DNA binding domain (CTD) on topo IV's ParC subunit. Biochemical studies show that the hinge stimulates topo IV by competing for a site on the CTD that normally represses activity on negatively supercoiled DNA, while complementation tests using mutants implicated in the interaction reveal that the cellular dependency on topo IV derives from a joint need for both strand passage and MukB binding. Interestingly, the configuration of the MukB.topo IV complex sterically disfavours intradimeric interactions, indicating that the proteins may form oligomeric arrays with one another, and suggesting a framework by which MukB and topo IV may collaborate during daughter chromosome disentanglement. | ||
| - | + | Structural basis for the MukB-topoisomerase IV interaction and its functional implications in vivo.,Vos SM, Stewart NK, Oakley MG, Berger JM EMBO J. 2013 Oct 4. doi: 10.1038/emboj.2013.218. PMID:24097060<ref>PMID:24097060</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | < | + | </div> |
| - | [[Category: Escherichia coli str. | + | <div class="pdbe-citations 4mn4" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Condensin|Condensin]] |
| - | [[Category: | + | *[[Topoisomerase 3D structures|Topoisomerase 3D structures]] |
| - | [[Category: | + | == References == |
| - | + | <references/> | |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
| + | [[Category: Escherichia coli str. K-12 substr. MG1655]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Berger JM]] | ||
| + | [[Category: Oakley MG]] | ||
| + | [[Category: Stewart NK]] | ||
| + | [[Category: Vos SM]] | ||
Current revision
Structural Basis for the MukB-topoisomerase IV Interaction
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