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2pex

From Proteopedia

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[[Image:2pex.jpg|left|200px]]<br /><applet load="2pex" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2pex, resolution 1.900&Aring;" />
 
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'''Structure of reduced C22S OhrR from Xanthamonas Campestris'''<br />
 
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==Overview==
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==Structure of reduced C22S OhrR from Xanthamonas Campestris==
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The Xanthomonas campestris transcription regulator OhrR contains a reactive cysteine residue (C22) that upon oxidation by organic hydroperoxides (OHPs) forms an intersubunit disulphide bond with residue C127'. Such modification induces the expression of a peroxidase that reduces OHPs to their less toxic alcohols. Here, we describe the structures of reduced and OHP-oxidized OhrR, visualizing the structural mechanism of OHP induction. Reduced OhrR takes a canonical MarR family fold with C22 and C127' separated by 15.5 A. OHP oxidation results in the disruption of the Y36'-C22-Y47' interaction network and dissection of helix alpha5, which then allows the 135 degrees rotation and 8.2 A translation of C127', formation of the C22-C127' disulphide bond, and alpha6-alpha6' helix-swapped reconfiguration of the dimer interface. These changes result in the 28 degrees rigid body rotations of each winged helix-turn-helix motif and DNA dissociation. Similar effector-induced rigid body rotations are expected for most MarR family members.
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<StructureSection load='2pex' size='340' side='right'caption='[[2pex]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2pex]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PEX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pex OCA], [https://pdbe.org/2pex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pex RCSB], [https://www.ebi.ac.uk/pdbsum/2pex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pex ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q93R11_XANCH Q93R11_XANCH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pe/2pex_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pex ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2PEX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris] with <scene name='pdbligand=FMT:'>FMT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PEX OCA].
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*[[Organic hydroperoxide resistance protein|Organic hydroperoxide resistance protein]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural mechanism of organic hydroperoxide induction of the transcription regulator OhrR., Newberry KJ, Fuangthong M, Panmanee W, Mongkolsuk S, Brennan RG, Mol Cell. 2007 Nov 30;28(4):652-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18042459 18042459]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Xanthomonas campestris]]
[[Category: Xanthomonas campestris]]
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[[Category: Brennan, R G.]]
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[[Category: Brennan RG]]
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[[Category: Newberry, K J.]]
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[[Category: Newberry KJ]]
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[[Category: FMT]]
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[[Category: transcription regulator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:28:43 2008''
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Current revision

Structure of reduced C22S OhrR from Xanthamonas Campestris

PDB ID 2pex

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