4m67

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{{STRUCTURE_4m67| PDB=4m67 | SCENE= }}
 
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===Crystal structure of the human MLKL kinase-like domain===
 
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{{ABSTRACT_PUBMED_24095729}}
 
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==Function==
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==Crystal structure of the human MLKL kinase-like domain==
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[[http://www.uniprot.org/uniprot/MLKL_HUMAN MLKL_HUMAN]] Required for the execution of programmed necrosis.<ref>PMID:22265414</ref>
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<StructureSection load='4m67' size='340' side='right'caption='[[4m67]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4m67]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M67 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m67 OCA], [https://pdbe.org/4m67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m67 RCSB], [https://www.ebi.ac.uk/pdbsum/4m67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m67 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MLKL_HUMAN MLKL_HUMAN] Required for the execution of programmed necrosis.<ref>PMID:22265414</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RIP3 is an essential upstream kinase in necroptosis. The pseudokinase MLKL functions as a substrate of RIP3 to mediate downstream signaling. The molecular mechanism by which RIP3 recognizes and phosphorylates MLKL remains unknown. Here, we report the crystal structures of the mouse RIP3 kinase domain, the MLKL kinase-like domain, and a binary complex between the two. Both RIP3 and MLKL adopt the canonical kinase fold. Free RIP3 exists in an active conformation, whereas MLKL-bound RIP3 is stabilized by AMP-PNP to adopt an inactive conformation. The formation of the RIP3-MLKL complex, involving their respective N- and C-lobes, is accompanied by pronounced conformational changes of the alphaC helix and activation loop in RIP3 and the corresponding structural elements in MLKL. RIP3-mediated MLKL phosphorylation, though important for downstream signaling, is dispensable for stable complex formation between RIP3 and MLKL. Our study serves as a framework for mechanistic understanding of RIP3-mediated necroptotic signaling.
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==About this Structure==
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Structural Insights into RIP3-Mediated Necroptotic Signaling.,Xie T, Peng W, Yan C, Wu J, Gong X, Shi Y Cell Rep. 2013 Oct 17;5(1):70-8. doi: 10.1016/j.celrep.2013.08.044. Epub 2013 Oct, 3. PMID:24095729<ref>PMID:24095729</ref>
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[[4m67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M67 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024095729</ref><references group="xtra"/><references/>
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</div>
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<div class="pdbe-citations 4m67" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Peng, W.]]
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[[Category: Large Structures]]
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[[Category: Shi, Y.]]
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[[Category: Peng W]]
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[[Category: Wu, J.]]
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[[Category: Shi Y]]
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[[Category: Xie, T.]]
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[[Category: Wu J]]
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[[Category: Yan, C.]]
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[[Category: Xie T]]
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[[Category: A substrate of rip3]]
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[[Category: Yan C]]
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[[Category: Kinase-like]]
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[[Category: Rip3]]
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[[Category: Signaling protein]]
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Current revision

Crystal structure of the human MLKL kinase-like domain

PDB ID 4m67

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