2psf

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[[Image:2psf.gif|left|200px]]<br /><applet load="2psf" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2psf, resolution 1.40&Aring;" />
 
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'''Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis'''<br />
 
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==Overview==
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==Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis==
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Due to its ability to emit light, the luciferase from Renilla reniformis (RLuc) is widely employed in molecular biology as a reporter gene in cell culture experiments and small animal imaging. To accomplish this bioluminescence, the 37-kDa enzyme catalyzes the degradation of its substrate coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light. We successfully crystallized a stabilized variant of this important protein (RLuc8) and herein present the first structures for any coelenterazine-using luciferase. These structures are based on high-resolution data measured to 1.4 A and demonstrate a classic alpha/beta-hydrolase fold. We also present data of a coelenteramide-bound luciferase and reason that this structure represents a secondary conformational form following shift of the product out of the primary active site. During the course of this work, the structure of the luciferase's accessory green fluorescent protein (RrGFP) was also determined and shown to be highly similar to that of Aequorea victoria GFP.
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<StructureSection load='2psf' size='340' side='right'caption='[[2psf]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2psf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PSF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2psf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2psf OCA], [https://pdbe.org/2psf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2psf RCSB], [https://www.ebi.ac.uk/pdbsum/2psf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2psf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LUCI_RENRE LUCI_RENRE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/2psf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2psf ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2PSF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Active as [http://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSF OCA].
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*[[Luciferase 3D structures|Luciferase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis., Loening AM, Fenn TD, Gambhir SS, J Mol Biol. 2007 Dec 7;374(4):1017-28. Epub 2007 Oct 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17980388 17980388]
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[[Category: Large Structures]]
[[Category: Renilla reniformis]]
[[Category: Renilla reniformis]]
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[[Category: Renilla-luciferin 2-monooxygenase]]
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[[Category: Fenn TD]]
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[[Category: Single protein]]
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[[Category: Gambhir SS]]
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[[Category: Fenn, T D.]]
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[[Category: Loening AM]]
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[[Category: Gambhir, S S.]]
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[[Category: Loening, A M.]]
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[[Category: alpha/beta-hydrolase]]
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[[Category: luciferase]]
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[[Category: oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:32:31 2008''
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Current revision

Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis

PDB ID 2psf

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