|
|
| (4 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:2pzo.jpg|left|200px]]<br /><applet load="2pzo" size="350" color="white" frame="true" align="right" spinBox="true" | + | #REDIRECT [[3e2u]] This PDB entry is obsolete and replaced by 3e2u |
| - | caption="2pzo, resolution 2.60Å" />
| + | |
| - | '''Crystal structure of the zinc-knuckle 2 domain of human CLIP-170 in complex with CAP-Gly domain of human Dynactin-1 (p150-Glued)'''<br />
| + | |
| - | | + | |
| - | ==Overview==
| + | |
| - | In all eukaryotes, CAP-Gly proteins control important cellular processes. The molecular mechanisms underlying the functions of CAP-Gly domains, however, are still poorly understood. Here we use the complex formed between the CAP-Gly domain of p150(glued) and the C-terminal zinc knuckle of CLIP170 as a model system to explore the structure-function relationship of CAP-Gly-mediated protein interactions. We demonstrate that the conserved GKNDG motif of CAP-Gly domains is responsible for targeting to the C-terminal EEY/F sequence motifs of CLIP170, EB proteins and microtubules. The CAP-Gly-EEY/F interaction is essential for the recruitment of the dynactin complex by CLIP170 and for activation of CLIP170. Our findings define the molecular basis of CAP-Gly domain function, including the tubulin detyrosination-tyrosination cycle. They further establish fundamental roles for the interaction between CAP-Gly proteins and C-terminal EEY/F sequence motifs in regulating complex and dynamic cellular processes.
| + | |
| - | | + | |
| - | ==Disease==
| + | |
| - | Known diseases associated with this structure: Amyotrophic lateral sclerosis, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=601143 601143]], Neuropathy, distal hereditary motor, type VIIB OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=601143 601143]]
| + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 2PZO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PZO OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | Structure-function relationship of CAP-Gly domains., Weisbrich A, Honnappa S, Jaussi R, Okhrimenko O, Frey D, Jelesarov I, Akhmanova A, Steinmetz MO, Nat Struct Mol Biol. 2007 Oct;14(10):959-67. Epub 2007 Sep 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17828277 17828277]
| + | |
| - | [[Category: Homo sapiens]]
| + | |
| - | [[Category: Protein complex]]
| + | |
| - | [[Category: Honnappa, S.]]
| + | |
| - | [[Category: Steinmetz, M O.]]
| + | |
| - | [[Category: Weisbrich, A.]]
| + | |
| - | [[Category: ZN]]
| + | |
| - | [[Category: +tip protein complex structure]]
| + | |
| - | [[Category: autoinhibition]]
| + | |
| - | [[Category: clip-170/restin]]
| + | |
| - | [[Category: cytoskeleton associated protein]]
| + | |
| - | [[Category: dynactin]]
| + | |
| - | [[Category: p150glued]]
| + | |
| - | [[Category: protein binding]]
| + | |
| - | [[Category: structural protein microtubule binding]]
| + | |
| - | [[Category: zinc-knuckle]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:34:37 2008''
| + | |
